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Effects of dietary supplementation with macroalgae on sperm quality and antioxidant system in Senegalese sole

2024-09Journal article Open access
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dc.contributor.authorFélix de Azeredo Pinto e Melo, Francisca
dc.contributor.authorSilva, Nádia
dc.contributor.authorOliveira, Catarina
dc.contributor.authorCabrita, Elsa
dc.contributor.authorGavaia, Paulo
dc.date.accessioned2024-06-26T09:30:00Z
dc.date.available2024-06-26T09:30:00Z
dc.date.issued2024-09
dc.description.abstractWithin the efforts in the search for novel feed ingredients in aquaculture, macroalgae are being widely explored due to their diversity of bioactive compounds with considerable prophylactic and therapeutic potential. Plocamium cartilagineum and Sargassum vulgare, have the potential to positively impact fish health, mainly due to their antioxidant properties. The production of Senegalese sole (Solea senegalensis) still faces some constraints due to the low sperm quantity and quality of males from captivity, which is highly affected by oxidative stress. We hypothesized that the scavenger ability of P. cartilagineum and S. vulgare could ameliorate the reproductive performance of Senegalese sole males, once incorporated in the roodstock feeds. In this experiment, broodstock groups were fed with three different formulated experimental diets for 60 days: Control (Ctrl), a diet supplemented with 5% P. cartilagineum (Pc), and a diet supplemented with 5% S. vulgare (Sv). Fish were sampled twice to evaluate sperm quality using different techniques: sperm motility by Computer Assisted Sperm Analysis (CASA) software, and cell viability and Reactive Oxygen Species (ROS) by flow cytometer. At the final sampling, 6 fish per treatment were sacrificed and gonads were extracted to determine the relative expression of anti- and pro-apoptotic genes (bcl2b, bcl-x, mcl1b, badb, bax, boka), and genes involved in fish antioxidant system (sod3, cat, gpx, hsp70). Sex steroids were measured on blood plasma by ELISA. Specific growth rate (SGR) and feed efficiency were also evaluated at the end of the experiment. Senegalese sole breeders fed with Pc diet for 60 days showed enhanced sperm total motility and cell viability at the end of the experiment, when compared with the Ctrl diet. On the other hand, fish fed with Sv diet had lower spermatozoa intracellular ROS and upregulated gpx in the gonads. No differences were obtained in levels of sex steroids and gonadal apoptotic system after 60 days of feeding, however, the macroalgae species and the percentages of incorporation used proved to positively modulate different spermatozoa traits.eng
dc.description.sponsorshipMarie Sklodowska-Curie ITN BIOMEDAQU H2020-MSCA-ITN/766347 The "Programa Crescimento Azul" (EEA grants project-BREEDFLAT) 4 PT-INNOVATION-0080; 57/2016/CP1361/CT0007
dc.identifier.doi10.1016/j.aquaculture.2024.741069
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/25543
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationImprovement of Senegalese sole sperm quality through the action of melatonin as an antioxidant
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofAquaculture
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPlocamium cartilagineum
dc.subjectSargassum vulgare
dc.subjectFeed supplementation
dc.subjectSolea senegalensis reproduction
dc.subjectF1 broodstock
dc.titleEffects of dietary supplementation with macroalgae on sperm quality and antioxidant system in Senegalese sole
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleImprovement of Senegalese sole sperm quality through the action of melatonin as an antioxidant
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F148280%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.startPage741069
oaire.citation.titleAquaculture
oaire.citation.volume590
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameFélix de Azeredo Pinto e Melo
person.familyNameSilva
person.familyNameOliveira
person.familyNameCabrita
person.familyNameGavaia
person.givenNameFrancisca
person.givenNameNádia
person.givenNameCatarina
person.givenNameElsa
person.givenNamePaulo
person.identifier.ciencia-idAC1C-FB89-E69C
person.identifier.ciencia-id5D1F-6F3A-A1C2
person.identifier.ciencia-id0816-30C5-51AC
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0001-9881-4421
person.identifier.orcid0000-0002-6679-761X
person.identifier.orcid0000-0003-3864-8841
person.identifier.orcid0000-0002-9582-1957
person.identifier.ridE-8523-2012
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id10244362100
person.identifier.scopus-author-id35433190500
person.identifier.scopus-author-id26645697000
person.identifier.scopus-author-id6507104377
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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