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Improvement of carotenoid extraction from a recently isolated, robust microalga, Tetraselmis sp. CTP4 (chlorophyta)

2020Journal article Open access
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dc.contributor.authorLisa Schueler, Lisa M. Schueler
dc.contributor.authorKatkam, Dr. Gangadhar N.
dc.contributor.authorDuarte, Paulo A. S.
dc.contributor.authorPlacines, Chloé
dc.contributor.authorMolina-Márquez, Ana María
dc.contributor.authorLéon-Bañares, Rosa
dc.contributor.authorSousa, Vânia S.
dc.contributor.authorVarela, João
dc.contributor.authorBarreira, Luísa
dc.date.accessioned2020-07-31T14:13:58Z
dc.date.available2021-05-01T00:30:30Z
dc.date.issued2020
dc.description.abstractIn recent years, there has been increasing consumer interest in carotenoids, particularly of marine sustainable origin with applications in the food, cosmeceutical, nutritional supplement and pharmaceutical industries. For instance, microalgae belonging to the genus Tetraselmis are known for their biotechnologically relevant carotenoid profile. The recently isolated marine microalgal strain Tetraselmis sp. CTP4 is a fast-growing, robust industrial strain, which has successfully been produced in 100-m3 photobioreactors. However, there are no reports on total carotenoid contents from this strain belonging to T. striata/convolutae clade. Although there are several reports on extraction methods targeting chlorophytes, extraction depends on the strength of cell coverings, solvent polarity and the nature of the targeted carotenoids. Therefore, this article evaluates different extraction methods targeting Tetraselmis sp. CTP4, a strain known to contain a mechanically resistant theca. Here, we propose a factorial experimental design to compare extraction of total carotenoids from wet and freeze-dried microalgal biomass using four different solvents (acetone, ethanol, methanol or tetrahydrofuran) in combination with two types of mechanical cell disruption (glass beads or dispersion). The extraction efficiency of the methods was assessed by pigment contents and profiles present in the extracts. Extraction of wet biomass by means of glass bead-assisted cell disruption using tetrahydrofuran yielded the highest amounts of lutein and β-carotene (622 ± 40 and 618 ± 32 µg g-1 DW, respectively). Although acetone was slightly less efficient than tetrahydrofuran, it is preferable due to its lower costs and toxicity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00449-019-02273-9pt_PT
dc.identifier.issn1615-7591
dc.identifier.urihttp://hdl.handle.net/10400.1/14562
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.subjectMarine microalgaept_PT
dc.subjectGlass beadspt_PT
dc.subjectLuteinpt_PT
dc.subjectRP-HPLCpt_PT
dc.subjectWet biomasspt_PT
dc.titleImprovement of carotenoid extraction from a recently isolated, robust microalga, Tetraselmis sp. CTP4 (chlorophyta)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage796pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage785pt_PT
oaire.citation.titleBioprocess and Biosystems Engineeringpt_PT
oaire.citation.volume43pt_PT
person.familyNameSchüler
person.familyNameKatkam
person.familyNamePlacines
person.familyNameVarela
person.familyNameBarreira
person.givenNameLisa
person.givenNameDr. Gangadhar
person.givenNameChloé
person.givenNameJoão
person.givenNameLuísa
person.identifier.ciencia-idE01F-9B43-4F24
person.identifier.ciencia-idB91F-5FD0-CB1C
person.identifier.ciencia-id6D1A-17E5-1400
person.identifier.orcid0000-0003-0961-5397
person.identifier.orcid0000-0002-5368-5475
person.identifier.orcid0000-0001-8550-5099
person.identifier.orcid0000-0003-3101-693X
person.identifier.orcid0000-0002-4077-855X
person.identifier.ridM-4677-2013
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id11638783300
person.identifier.scopus-author-id55741416000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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