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In vitro anti-trypanosoma cruzi activity of halophytes from Southern Portugal reloaded: a special focus on Sea Fennel (Crithmum maritimum L.)

2021-11Journal article Open access
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dc.contributor.authorPereira, Catarina Guerreiro
dc.contributor.authorMoraes, Carolina Borsoi
dc.contributor.authorFranco, Caio H.
dc.contributor.authorFeltrin, Clarissa
dc.contributor.authorGrougnet, Raphaël
dc.contributor.authorBarbosa, Euzébio Guimarães
dc.contributor.authorPanciera, Michele
dc.contributor.authorCorreia, Carlos Roque D.
dc.contributor.authorRodrigues, Maria Joao
dc.contributor.authorL, Custódio
dc.date.accessioned2021-12-13T13:56:24Z
dc.date.available2021-12-13T13:56:24Z
dc.date.issued2021-11
dc.description.abstractMarine halophytes are an outstanding reservoir of natural products and several species have anti-infectious traditional uses. However, reports about their potential use against neglected tropical ailments, such as Chagas disease, are scarce. This work evaluated for the first time the in vitro anti-Trypanosoma cruzi activity of extracts from the aromatic and medicinal species Helichrysum italicum subsp. picardii (Boiss. & Reut.) Franco (Asteraceae, everlasting) and Crithmum maritimum L. (Apiaceae, sea fennel). For that purpose, decoctions, tinctures, and essential oils from everlasting’s flowers and sea fennel’s stems, leaves, and flowers were tested against intracellular amastigotes of two T. cruzi strains. The extract from the sea fennel flower decoction displayed significant anti-trypanosomal activity and no toxicity towards the host cell (EC50 = 17.7 µg/mL, selectivity index > 5.65). Subsequent fractionation of this extract afforded 5 fractions that were re-tested in the same model of anti-parasitic activity. Fraction 1 was the most active and selective (EC50 = 0.47 µg/mL, selectivity index = 59.6) and was submitted to preparative thin-layer chromatography. One major compound was identified, falcarindiol, which was likely the one responsible for the observed antitrypanosomal activity. This was confirmed using a commercially sourced molecule. Target-fishing studies showed falcarindiol as a ligand of T. cruzi spermidine synthase, pointing to a potential enzyme-inhibiting anti-trypanosomal mechanism of action. Overall, this work shows that sea fennel can provide effective anti-parasitic molecule(s) with potential pharmacological applications in the treatment of CD.pt_PT
dc.description.sponsorshipFA-05-2017-028; 141635/2014-2; 2013/25613-5
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/plants10112235pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/17379
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMarine halophytespt_PT
dc.subjectChagas diseasept_PT
dc.subjectTrypanosoma cruzipt_PT
dc.subjectNeglected tropical diseasespt_PT
dc.subjectCrithmum maritimumpt_PT
dc.subjectFalcarindiolpt_PT
dc.titleIn vitro anti-trypanosoma cruzi activity of halophytes from Southern Portugal reloaded: a special focus on Sea Fennel (Crithmum maritimum L.)pt_PT
dc.title.alternativeIn vitro anti-trypanosoma cruzi atividade de halophytes do Sul de Portugal recarregado: um foco especial em Erva-Do Mar (Crithmum maritimum L.)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00425%2F2017%2FCP1391%2FCT0001/PT
oaire.citation.issue11pt_PT
oaire.citation.startPage2235pt_PT
oaire.citation.titlePlantspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2017
person.familyNameGuerreiro Pereira
person.familyNameRodrigues
person.familyNameCustódio
person.givenNameCatarina Alexandra
person.givenNameMaria João
person.givenNameLuísa
person.identifierNmxO5SIAAAAJ
person.identifierR-004-VNG
person.identifier.ciencia-id4512-3435-689C
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.orcid0000-0002-1131-8773
person.identifier.orcid0000-0001-8732-710X
person.identifier.orcid0000-0003-4338-7703
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id56031608100
person.identifier.scopus-author-id15831018900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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