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Quantification of prolactin (PRL) and PRL receptor messenger RNA in gilthead Seabream (Spares aurata) after treatment with Estradiol-17 beta

2003-02Journal article Restricted access
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dc.contributor.authorCavaco, J. E. B.
dc.contributor.authorSantos, C. R. A.
dc.contributor.authorIngleton, P. M.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-05-20T10:04:48Z
dc.date.available2014-05-20T10:04:48Z
dc.date.issued2003-02
dc.date.updated2014-05-13T10:22:26Z
dc.description.abstractProlactin (PRL) in fish is considered to be an osmoregulatory hormone, although some studies suggest that it may influence the production of steroid hormones in the gonads. The objective of the present study was to establish if PRL is involved in reproduction of the gilthead seabream—a protandrous hermaphrodite. Adult and juvenile gilthead seabream received implants of estradiol-17b (E2) for 1 wk during the breeding season, and the mRNA expressions of PRL and PRL receptor (sbPRLR) were determined. Northern blot analysis revealed a single pituitary PRL transcript, the expression of which was significantly reduced by E2 treatment in adults but significantly increased in juvenile fish. In adult gonads, four sbPRLR transcripts of 1.1, 1.3, 1.9, and 2.8 kilobases were observed. A competitive reverse transcription-polymerase chain reaction was developed and used to determine how E2 treatment alters expression of the gonadal sbPRLR gene. Seabream PRLR was detectable in all samples analyzed by this assay. Levels of sbPRLR mRNA increased significantly (50-fold) after E2 treatment in adults, but a 24-fold decrease was measured in juveniles. Immunohistochemistry using specific polyclonal antibodies raised against an oligopeptide from the extracellular domain of sbPRLR detected the receptor in spermatogonia and oocytes. Taken together, the preceding results suggest that in the seabream, PRL may act on both testis and ovary via its receptor and that the stage of maturity influences this process. The full characterization and relative importance of the different transcripts of sbPRLR in eliciting the action of PRL in the gonads remain to be elucidated.por
dc.identifier.citationCavaco, J. E. B.; Santos, C. R. A.; Ingleton, P. M.; Canario, A. V. M.; Power, D. M.Quantification of prolactin (PRL) and PRL receptor messenger RNA in gilthead Seabream (Spares aurata) after treatment with Estradiol-17 beta, Biology of Reproduction, 68, 2, 588-594, 2003.por
dc.identifier.doihttp://dx.doi.org/10.1095/biolreprod.102.009209
dc.identifier.issn0006-3363
dc.identifier.otherAUT: ACA00258; DPO00386
dc.identifier.urihttp://hdl.handle.net/10400.1/4004
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSociety for the Study of Reproductionpor
dc.relation.publisherversionhttp://www.biolreprod.org/search?submit=yes&submit=Submit&pubdate_year=&volume=&firstpage=&doi=10.1095%2Fbiolreprod.102.009209&mtgabsnum=&author1=&author2=&title=&andorexacttitle=and&titleabstract=&andorexacttitleabs=and&fulltext=&andorexactfulltext=and&fmonth=&fyear=&tmonth=&tyear=&format=standard&hits=10&sortspec=relevance&submit=yespor
dc.subjectProlactinpor
dc.subjectProlactin receptorpor
dc.subjectEstradiolpor
dc.subjectPituitarypor
dc.titleQuantification of prolactin (PRL) and PRL receptor messenger RNA in gilthead Seabream (Spares aurata) after treatment with Estradiol-17 betapor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage594por
oaire.citation.issue2por
oaire.citation.startPage588por
oaire.citation.titleBiology of Reproductionpor
oaire.citation.volume68por
person.familyNameCanario
person.familyNamePower
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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