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Effects of dietary lipid sources on hepatic nutritive contents, fatty acid composition and proteome of Nile tilapia (Oreochromis niloticus)

2019-02Journal article Restricted access
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dc.contributor.authorBoonanuntanasarn, Surintorn
dc.contributor.authorNakharuthai, Chatsirin
dc.contributor.authorSchrama, Denise
dc.contributor.authorDuangkaew, Rungsun
dc.contributor.authorRodrigues, Pedro M.
dc.date.accessioned2020-07-24T10:52:52Z
dc.date.available2020-07-24T10:52:52Z
dc.date.issued2019-02
dc.description.abstractDietary oils of varying fatty acid composition have been used in tilapia feeds; nevertheless, investigation of their effects on metabolism and physiological processes has been limited. Therefore, in this study, using proteomic technology, the effects of different dietary lipids (DLs) on hepatic lipid metabolism and physiological processes were investigaed in Nile tilapia (Oreochromis niloticus). Fish were fed with different DL, which included palm oil (PO), linseed oil (LO) and soybean oil (SBO) for 90 days. Growth performance appeared to be similar among the dietary group. Hepatic FA contents were reflected by DL. Dietary PO (source of saturated fatty acids; SFA) led to an increase in the hepatosomatic index as well as lipid and protein contents in liver. Dietary SBO (source of n-6 polyunsaturated fatty acids; n-6 PUFAs) increased hepatic carbohydrate contents, but decreased the hepatic protein. The proteomic analysis showed that these nutritive changes in the liver were mediated by several proteins involved in lipid, carbohydrate, and amino acid metabolism. Dietary SBO showed an increased accumulation in proteins related to oxidative stress, immune and inflammatory processes. Dietary LO (source of n-3 PUFAs) increased abundance of cytoskeleton-related proteins. qRT-PCR was performed to provide supportive information for the result of proteomic study. Similar effects of DLs on mRNA levels were observed for atp5b, krt18, and selenbp1. Combined together, dietary SFA could supply as energy reservoir for regular activites. Dietary n-3 and n-6 PUFAs led to induce vital metabolic and physiologic processes which would contribute to maintaining normal health and/or providing health-related benefits. Significance: Dietary SBO increased hepatic carbohydrate contents, but decreased the hepatic protein. Different dietary lipid led to alter the abundance of several proteins (i.e., DHRS1, ATP5B, PLA2G12B, APO, AMY2A, GRP78, PRSS1, FAH, and PRSS36) involved in lipid, carbohydrate, and amino acid metabolism. Dietary SBO showed an increased accumulation in proteins (i.e., QDPR, CABC1, and PRDX6) that respond to oxidative stress, suggesting that n-6 PUFAs induce oxidative conditions. Dietary SBO led to increase the accumulation of proteins (i.e., NITR26, NCCRP1, and LCN) involved in immune and inflammatory processes. Dietary LO increased the abundant levels of cytoskeleton-related proteins (i.e., ACTB, AHNAK, ERC2, KRT18, and RILP1). Other proteins (i.e., SELENBP1, FAM46C, and ANC1) involved in other physiological processes were also modulated by DL. qRT-PCR was performed to provide supportive information of proteomic study. Similar effects of DLs on mRNA levels were observed for atp5b, krt18, and selenbp1.
dc.description.sponsorshipThailand Research FundThailand Research Fund (TRF)
dc.description.sponsorshipSuranaree University of Technology [RSA5880052]
dc.description.sponsorshipHigher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission
dc.identifier.doi10.1016/j.jprot.2018.09.003
dc.identifier.issn1874-3919
dc.identifier.issn1876-7737
dc.identifier.urihttp://hdl.handle.net/10400.1/14415
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectWinter thermal-stress
dc.subjectRed hybrid tilapia
dc.subjectGrowth-performance
dc.subjectFish-oil
dc.subjectVegetable-oils
dc.subjectLinolenic acid
dc.subjectSparus-aurata
dc.subjectLiver
dc.subjectMetabolism
dc.subjectExpression
dc.titleEffects of dietary lipid sources on hepatic nutritive contents, fatty acid composition and proteome of Nile tilapia (Oreochromis niloticus)
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage222
oaire.citation.startPage208
oaire.citation.titleJournal of Proteomics
oaire.citation.volume192
person.familyNameSchrama
person.familyNameRodrigues
person.givenNameDenise
person.givenNamePedro
person.identifier159154
person.identifier.ciencia-id501C-AC69-68C0
person.identifier.orcid0000-0003-0274-5957
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridH-6511-2016
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id55755583400
person.identifier.scopus-author-id55107531000
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication00f4fa6e-b467-4d5c-83f2-a454f7b4bc71
relation.isAuthorOfPublicationc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72
relation.isAuthorOfPublication.latestForDiscoveryc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72

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