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Metabolic rate and rates of protein turnover in food-deprived cuttlefish, Sepia officinalis (Linnaeus 1758)

2016-04-06Journal article Restricted access
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dc.contributor.authorLamarre, Simon G.
dc.contributor.authorMacCormack, Tyson J.
dc.contributor.authorSykes, António V.
dc.contributor.authorHall, Jennifer R.
dc.contributor.authorSpeers-Roesch, Ben
dc.contributor.authorCallaghan, Neal I.
dc.contributor.authorDriedzic, William R.
dc.date.accessioned2018-11-09T16:44:33Z
dc.date.available2018-11-09T16:44:33Z
dc.date.issued2016-04-06
dc.description.abstractTo determine the metabolic response to food deprivation, cuttlefish (Sepia officinalis) juveniles were either fed, fasted (3 to 5 days food deprivation), or starved (12 days food deprivation). Fasting resulted in a decrease in triglyceride levels in the digestive gland, and after 12 days, these lipid reserves were essentially depleted. Oxygen consumption was decreased to 53% and NH4 excretion to 36% of the fed group following 3-5 days of food deprivation. Oxygen consumption remained low in the starved group, but NH4 excretion returned to the level recorded for fed animals during starvation. The fractional rate of protein synthesis of fasting animals decreased to 25% in both mantle and gill compared with fed animals and remained low in the mantle with the onset of starvation. In gill, however, protein synthesis rate increased to a level that was 45% of the fed group during starvation. In mantle, starvation led to an increase in cathepsin A-, B-, H-, and L-like enzyme activity and a 2.3-fold increase in polyubiquitin mRNA that suggested an increase in ubiquitin-proteasome activity. In gill, there was a transient increase in the polyubiquitin transcript levels in the transition from fed through fasted to the starved state and cathepsin A-, B-, H-, and L-like activity was lower in starved compared with fed animals. The response in gill appears more complex, as they better maintain rates of protein synthesis and show no evidence of enhanced protein breakdown through recognized catabolic processes.pt_PT
dc.description.sponsorshipSEPIATECH 31-03-05-FEP-2 Program PROMAR
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.1152/ajpregu.00459.2015pt_PT
dc.identifier.issn0363-6119
dc.identifier.urihttp://hdl.handle.net/10400.1/10924
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Physiological Societypt_PT
dc.relationTowards cephalopod aquaculture: cuttlefish as the spearhead species
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectDecapodiformespt_PT
dc.subjectEnergy Metabolismpt_PT
dc.subjectGillspt_PT
dc.subjectMetabolic Clearance Ratept_PT
dc.subjectOrgan Specificitypt_PT
dc.subjectStarvationpt_PT
dc.subjectFood Deprivationpt_PT
dc.subjectOxygen Consumptionpt_PT
dc.subjectProtein Biosynthesispt_PT
dc.subjectNH4 productionpt_PT
dc.subjectAmmonia quotientpt_PT
dc.subjectCathepsinpt_PT
dc.subjectTriglyceridept_PT
dc.subjectDigestive glandpt_PT
dc.subjectProteasomept_PT
dc.subjectPolyubiquitinpt_PT
dc.titleMetabolic rate and rates of protein turnover in food-deprived cuttlefish, Sepia officinalis (Linnaeus 1758)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTowards cephalopod aquaculture: cuttlefish as the spearhead species
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00576%2F2014%2FCP1217%2FCT0002/PT
oaire.citation.endPage1168pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage1160pt_PT
oaire.citation.titleAJP - Regulatory, Integrative and Comparative Physiologypt_PT
oaire.citation.volume310pt_PT
oaire.fundingStreamInvestigador FCT
person.familyNameSykes
person.givenNameAntónio
person.identifier107454
person.identifier.ciencia-id7510-6641-5A42
person.identifier.orcid0000-0002-5207-0612
person.identifier.ridC-3609-2012
person.identifier.scopus-author-id7102626426
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryb9d55f95-5de8-40cf-82a3-96ec98c91a55
relation.isProjectOfPublicationa7bbb7aa-912c-4d7f-933a-3905800d8dcf
relation.isProjectOfPublication.latestForDiscoverya7bbb7aa-912c-4d7f-933a-3905800d8dcf

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