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Sulfated polysaccharides in marine sponges: extraction methods and anti-HIV activity

2011-01Journal article Open access
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dc.contributor.authorEsteves, Ana I. S.
dc.contributor.authorNicolai, Marisa
dc.contributor.authorHumanes, Madalena
dc.contributor.authorGonçalves, João
dc.date.accessioned2018-12-07T14:53:10Z
dc.date.available2018-12-07T14:53:10Z
dc.date.issued2011-01
dc.description.abstractThe extraction, fractionation and HIV-1 inhibition potential of polysaccharides extracted from three species of marine sponges, Erylus discophorus, Cliona celata and Stelletta sp., collected in the Northeastern Atlantic, is presented in this work. The anti-HIV activity of 23 polysaccharide pellets and three crude extracts was tested. Crude extracts prepared from Erylus discophorus specimens were all highly active against HIV-1 (90 to 95% inhibition). Cliona celata pellets showed low polysaccharide content (bellow 38.5%) and almost no anti-HIV activity (<10% inhibition). Stelletta sp. pellets, although quite rich in polysaccharide (up to 97.3%), showed only modest bioactivity (<36% HIV-1 inhibition). Erylus discophorus pellets were among the richest in terms of polysaccharide content (up to 98%) and the most active against HIV-1 (up to 95% inhibition). Chromatographic fractionation of the polysaccharide pellet obtained from a specimen of Erylus discophorus (B161) yielded only modestly active fractions. However, we could infer that the active molecule is most probably a high molecular weight sulfated polysaccharide (>2000 kDa), whose mechanism is possibly preventing viral attachment and entry (fusion inhibitor).
dc.description.sponsorshipPortuguese Science and Technology Foundation (FCT) [POCTI/FCB/38185/2001, SFRH/BD/17874/2004]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.3390/md9010139
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.1/11384
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI Ag
dc.relationAnti-HIV activity products from marine sponges
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnti-Hiv-1 activity
dc.subjectNatural-products
dc.subjectIn-Vitro
dc.subjectCell-aggregation
dc.subjectAlgae
dc.subjectAgents
dc.subjectGp120
dc.subjectGlycoprotein
dc.subjectRecognition
dc.subjectInhibition
dc.titleSulfated polysaccharides in marine sponges: extraction methods and anti-HIV activity
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAnti-HIV activity products from marine sponges
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FFCB%2F38185%2F2001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F17874%2F2004/PT
oaire.citation.endPage153
oaire.citation.issue1
oaire.citation.startPage139
oaire.citation.titleMarine Drugs
oaire.citation.volume9
oaire.fundingStreamPOCI
oaire.fundingStreamSFRH
person.familyNameEsteves
person.givenNameAna
person.identifier293165
person.identifier.orcid0000-0002-9614-0635
person.identifier.scopus-author-id22950697100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication10676ea1-d3db-495f-85a9-71e2ebf3d9dc
relation.isAuthorOfPublication.latestForDiscovery10676ea1-d3db-495f-85a9-71e2ebf3d9dc
relation.isProjectOfPublication92e05136-82eb-44a6-84fc-be2d812fc3f5
relation.isProjectOfPublicationa08d98b3-2ae3-4209-9644-c0ca6744d8a5
relation.isProjectOfPublication.latestForDiscovery92e05136-82eb-44a6-84fc-be2d812fc3f5

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