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Your horse is a donkey! Identifying domesticated equids from Western Iberia using collagen fingerprinting

2023-01Journal article Open access
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dc.contributor.authorPaladugu, Roshan
dc.contributor.authorRichter, Kristine Korzow
dc.contributor.authorValente, Maria João
dc.contributor.authorGabriel, Sónia
dc.contributor.authorDetry, Cleia
dc.contributor.authorWarinner, Christina
dc.contributor.authorDias, Cristina Barrocas
dc.date.accessioned2023-02-09T14:56:54Z
dc.date.available2023-02-09T14:56:54Z
dc.date.issued2023-01
dc.description.abstractSkeletal remains of two equid species, Equus caballus (horse) and Equus asinus (donkey), have been found in archaeological contexts throughout Iberia since the Palaeolithic and Chalcolithic periods, respectively. These two species play different economic and cultural roles, and therefore it is important to be able to distinguish between the two species to better understand their relative importance in the past human societies. The most reliable morphological features for distinguishing between the two domesticated equids are based on cranial measure-ments and tooth enamel folds, leading to only a small percentage of archaeological remains that can be identified to species. Ancient DNA (aDNA) analysis can be used to reliably distinguish the two equids, but it can be cost prohibitive to apply to large assemblages, and aDNA preservation of non-cranial elements is often low. Collagen peptide mass fingerprinting by matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry, also known as zooarchaeology by mass spectrometry (ZooMS), is a minimally destructive and cost-effective alternative to aDNA analysis for taxonomic determination. However, current ZooMS markers lack resolution below the genus level Equus. In this paper, we report a novel ZooMS peptide marker that reliably distinguishes between horses and donkeys using the enzyme chymotrypsin. We apply this peptide marker to taxonomically identify bones from the Iberian Peninsula ranging from the Iron Age to the Late Modern Period. The peptide biomarker has the potential to facilitate the collection of morphological data for zooarchaeological studies of equids in Iberia and throughout Eurasia and Africa.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jas.2022.105696pt_PT
dc.identifier.eissn1095-9238
dc.identifier.urihttp://hdl.handle.net/10400.1/19038
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationEuropean Doctorate in ARchaeological and Cultural Heritage MATerials science
dc.relationCultures of dairying: gene-culture-microbiome evolution and the ancient invention of dairy foods
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPeptide mass fingerprintingpt_PT
dc.subjectZooarchaeologypt_PT
dc.subjectBonept_PT
dc.subjectPalaeontologypt_PT
dc.subjectArchaeologypt_PT
dc.subjectZooMSpt_PT
dc.titleYour horse is a donkey! Identifying domesticated equids from Western Iberia using collagen fingerprintingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEuropean Doctorate in ARchaeological and Cultural Heritage MATerials science
oaire.awardTitleCultures of dairying: gene-culture-microbiome evolution and the ancient invention of dairy foods
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/766311/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/804884/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FHAR-ARQ%2F4909%2F2020/PT
oaire.citation.startPage105696pt_PT
oaire.citation.titleJournal of Archaeological Sciencept_PT
oaire.citation.volume149pt_PT
oaire.fundingStreamH2020
oaire.fundingStreamH2020
oaire.fundingStream3599-PPCDT
person.familyNameValente
person.givenNameMaria João
person.identifier.ciencia-id271E-1935-B059
person.identifier.orcid0000-0002-6137-5995
person.identifier.ridB-3313-2017
person.identifier.scopus-author-id54382399000
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery4b396efe-da81-4db5-82bb-aadc95a0eecd
relation.isProjectOfPublication293e0a54-8e0a-4ca8-9853-44fddfd2b826
relation.isProjectOfPublication044a48a5-14d6-4e70-bc16-93ef5932c6de
relation.isProjectOfPublication475f0c35-1ff9-4d9e-9d9b-aba575347a11
relation.isProjectOfPublication.latestForDiscovery293e0a54-8e0a-4ca8-9853-44fddfd2b826

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