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A guide to the use of bioassays in exploration of natural resources

2024Journal article Open access
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dc.contributor.authorSabotič, Jerica
dc.contributor.authorBayram, Engin
dc.contributor.authorEzra, David
dc.contributor.authorGaudêncio, Susana P.
dc.contributor.authorHaznedaroğlu, Berat Z.
dc.contributor.authorJanež, Nika
dc.contributor.authorKtari, Leila
dc.contributor.authorLuganini, Anna
dc.contributor.authorMandalakis, Manolis
dc.contributor.authorSafarik, Ivo
dc.contributor.authorSimes, Dina
dc.contributor.authorStrode, Evita
dc.contributor.authorToruńska-Sitarz, Anna
dc.contributor.authorVaramogianni-Mamatsi, Despoina
dc.contributor.authorVarese, Giovanna Cristina
dc.contributor.authorVasquez, Marlen I.
dc.date.accessioned2024-03-22T11:55:52Z
dc.date.available2024-03-22T11:55:52Z
dc.date.issued2024
dc.description.abstractBioassays are the main tool to decipher bioactivities from natural resources thus their selection and quality are critical for optimal bioprospecting. They are used both in the early stages of compounds isolation/purification/ identification, and in later stages to evaluate their safety and efficacy. In this review, we provide a comprehensive overview of the most common bioassays used in the discovery and development of new bioactive compounds with a focus on marine bioresources. We present a comprehensive list of practical considerations for selecting appropriate bioassays and discuss in detail the bioassays typically used to explore antimicrobial, antibiofilm, cytotoxic, antiviral, antioxidant, and anti-ageing potential. The concept of quality control and bioassay validation are introduced, followed by safety considerations, which are critical to advancing bioactive compounds to a higher stage of development. We conclude by providing an application-oriented view focused on the development of pharmaceuticals, food supplements, and cosmetics, the industrial pipelines where currently known marine natural products hold most potential. We highlight the importance of gaining reliable bioassay results, as these serve as a starting point for application -based development and further testing, as well as for consideration by regulatory authorities.pt_PT
dc.description.sponsorshipAAC n◦ 41/ALG/2020 - Project n◦ 072583 – NUTRISAFEpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.biotechadv.2024.108307pt_PT
dc.identifier.issn0734-9750
dc.identifier.urihttp://hdl.handle.net/10400.1/20537
dc.language.isoporpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBioassay selectionpt_PT
dc.subjectBioactivitypt_PT
dc.subjectNatural productspt_PT
dc.subjectDrug discoverypt_PT
dc.subjectBlue biotechnologypt_PT
dc.subjectBioactivity-guided purificationpt_PT
dc.subjectValidationpt_PT
dc.subjectPreclinical trialspt_PT
dc.subjectBiodiscoverypt_PT
dc.titleA guide to the use of bioassays in exploration of natural resourcespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.startPage108307pt_PT
oaire.citation.titleBiotechnology Advancespt_PT
oaire.citation.volume71pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSimes
person.givenNameDina
person.identifier.orcid0000-0002-5145-4753
person.identifier.ridN-2789-2014
person.identifier.scopus-author-id7201884723
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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