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Functional analysis of the mouse Nodal antagonist, Cerl2, during left-right axis formation

2010-08Conference object Open access
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dc.contributor.authorInacio, Jose M
dc.contributor.authorMarques, Sara
dc.contributor.authorBelo, José A.
dc.date.accessioned2018-12-07T14:52:44Z
dc.date.available2018-12-07T14:52:44Z
dc.date.issued2010-08
dc.description.abstractAlthough recently our understanding of how the LR asymmetry is generated in vertebrate embryos has seen rapid progress, many important questions remain to be explained. In mouse embryos, the leftward flow of the extra-embryonic fluid in the node cavity, called nodal flow, seems to be the symmetry-breaking event. However, it is not yet know how this flow functions or how the asymmetric signal(s) generated in the node is/are transferred to the lateral plate mesoderm. The mouse gene cerberus-like2(cerl2) encodes a 20-kDa protein with a predicted signal peptide sequence and a cysteine-rich domain (CRD) containing nine cysteines characteristic of the Cerberus/DAN family. Whole-mount in situ hybridization studies showed that cerl2 transcripts could be first detected in a horseshoe-shaped expression pattern in the perinodal region of the mouse embryo (E7.0), resembling Nodal expression at this stage. At stage E7.5, expression of cerl2 begins to decrease in intensity on the left side, and by early somitogenesis (E8.0), it can be strongly detected in the right side of the node, assuming a complementary expression pattern to that observed in Nodal. Furthermore, it was shown that Cerl2 activity is upstream of the Nodal receptor inhibiting Nodal and its downstream targets. A physical interaction between these two proteins exists, which suggests that Cerl2 is a secreted Nodal antagonist. Here, to elucidate the role of Cerl2 protein in the early events of symmetry breaking the functional activity of this Nodal antagonist will be discussed.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.ydbio.2010.05.209
dc.identifier.issn0012-1606
dc.identifier.urihttp://hdl.handle.net/10400.1/11181
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAcademic Press Inc Elsevier Science
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleFunctional analysis of the mouse Nodal antagonist, Cerl2, during left-right axis formation
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceAlbuquerque, NM
oaire.citation.endPage465
oaire.citation.issue1
oaire.citation.startPage465
oaire.citation.titleDevelopmental Biology
oaire.citation.title69th Annual Meeting of the Society-for-Developmental-Biology/Japanese-Society-of-Developmental-Biologists
oaire.citation.volume344
person.familyNameInacio
person.familyNameMarques
person.familyNameBelo
person.givenNameJose M
person.givenNameSara
person.givenNameJosé A.
person.identifier.ciencia-idB810-3281-8D8C
person.identifier.ciencia-idBB17-0F5D-A4FB
person.identifier.ciencia-idBF13-08E9-25E6
person.identifier.orcid0000-0002-9790-8335
person.identifier.orcid0000-0001-6784-5612
person.identifier.orcid0000-0001-7384-0949
person.identifier.ridO-1455-2018
person.identifier.ridF-4444-2012
person.identifier.scopus-author-id6604046494
person.identifier.scopus-author-id6602141392
rcaap.rightsopenAccess
rcaap.typeconferenceObject
relation.isAuthorOfPublicationd1a81b33-a919-48ab-94b2-0b0418552245
relation.isAuthorOfPublication20dd7142-5b7a-465f-8943-a6ac33b91df1
relation.isAuthorOfPublication4bebeb48-6fac-41a9-8363-f849def5e019
relation.isAuthorOfPublication.latestForDiscoveryd1a81b33-a919-48ab-94b2-0b0418552245

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