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Genomics-metabolomics profiling disclosed marine Vvbrio spartinae 3.6 as a producer of a new branched side chain prodigiosin

2020-05Journal article Restricted access
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dc.contributor.authorVitale, Giovanni Andrea
dc.contributor.authorSciarretta, Martina
dc.contributor.authorEsposito, Fortunato Palma
dc.contributor.authorJanuary, Grant Garren
dc.contributor.authorGiaccio, Marianna
dc.contributor.authorBunk, Boyke
dc.contributor.authorSproeer, Cathrin
dc.contributor.authorBajerski, Felizitas
dc.contributor.authorPower, Deborah
dc.contributor.authorFesta, Carmen
dc.contributor.authorMonti, Maria Chiara
dc.contributor.authorD'Auria, Maria Valeria
dc.contributor.authorde Pascale, Donatella
dc.date.accessioned2021-06-24T11:35:34Z
dc.date.available2021-06-24T11:35:34Z
dc.date.issued2020-05
dc.description.abstractA wide range of prescreening tests for antimicrobial activity of 59 bacterial isolates from sediments of Ria Formosa Lagoon (Algarve, Portugal) disclosed Vibrio spartinae 3.6 as the most active antibacterial producing strain. This bacterial strain, which has not previously been submitted for chemical profiling, was subjected to de novo whole genome sequencing, which aided in the discovery and elucidation of a prodigiosin biosynthetic gene cluster that was predicted by the bioinformatic tool KEGG BlastKoala. Comparative genomics led to the identification of a new membrane di-iron oxygenase-like enzyme, annotated as Vspart_02107, which is likely to be involved in the biosynthesis of cycloprodigiosin and analogues. The combined genomicsmetabolomics profiling of the strain led to the isolation and identification of one new branched-chain prodigiosin (5) and to the detection of two new cyclic forms. Furthermore, the evaluation of the minimum inhibitory concentrations disclosed the major prodigiosin as very effective against multi-drug-resistant pathogens including Stenotrophomonas maltophilia, a clinical isolate of Listeria monocytogenes, as well as some human pathogens reported by the World Health Organization as prioritized targets.
dc.description.sponsorshipH2020-MSCA-RISE Ocean Medicines [GA 690944]
dc.description.sponsorshipH2020-MSCA-ITN-ETN MarPipe [GA 721421]
dc.description.sponsorshipMinistero dell'Universita e della Ricerca (MIUR) PRIN 2017 Ministry of Education, Universities and Research (MIUR) [2017A95NCJ]
dc.description.sponsorshipFCT-Foundation for Science and TechnologyPortuguese Foundation for Science and Technology [UID/Multi/04326/2019]
dc.description.sponsorshipoperational programs CRESC Algarve 2020 and COMPETE 2020 through project EMBRC.PT [ALG-01-0145-FEDER-022121]
dc.description.sponsorshipEuropean UnionEuropean Commission [730984]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1021/acs.jnatprod.9b01159
dc.identifier.issn0163-3864
dc.identifier.urihttp://hdl.handle.net/10400.1/16480
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Chemical Society
dc.relationOcean Medicines
dc.relationImproving the flow in the pipeline of the next generation of marine biodiscovery scientists
dc.relationCentre of Marine Sciences
dc.relationAssociation of European Marine Biological Laboratories Expanded
dc.subject.otherPlant Sciences;
dc.titleGenomics-metabolomics profiling disclosed marine Vvbrio spartinae 3.6 as a producer of a new branched side chain prodigiosin
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleOcean Medicines
oaire.awardTitleImproving the flow in the pipeline of the next generation of marine biodiscovery scientists
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleAssociation of European Marine Biological Laboratories Expanded
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/690944/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/721421/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/730984/EU
oaire.citation.endPage1504
oaire.citation.issue5
oaire.citation.startPage1495
oaire.citation.titleJournal of Natural Products
oaire.citation.volume83
oaire.fundingStreamH2020
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamH2020
person.familyNamePower
person.givenNameDeborah Mary
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccess
rcaap.typearticle
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