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Improved production of lutein and β-carotene by thermal and light intensity upshifts in the marine microalga Tetraselmis sp. CTP4

dc.contributor.authorSchüler, Lisa Maylin
dc.contributor.authorSantos, Tamara
dc.contributor.authorPereira, Hugo
dc.contributor.authorDuarte, Paulo
dc.contributor.authorKatkam, Dr. Gangadhar N.
dc.contributor.authorFlorindo, Claudia
dc.contributor.authorSchulze, Peter S.C.
dc.contributor.authorBarreira, Luísa
dc.contributor.authorVarela, João
dc.date.accessioned2020-02-17T17:19:25Z
dc.date.available2020-02-17T17:19:25Z
dc.date.issued2020
dc.description.abstractThe industrial microalga Tetraselmis sp. CTP4 is a promising candidate for aquaculture feed, novel food, cosmeceutical and nutraceutical due to its balanced biochemical profile. To further upgrade its biomass value, carotenogenesis was investigated by testing four environmental factors, namely temperature, light intensity, salinity and nutrient availability over different growth stages. The most important factor for carotenoid induction in this species is a sufficient supply of nitrates leading to an exponential growth of the cells. Furthermore, high temperatures of over 30 degrees C compared to lower temperatures (10 and 20 degrees C) induced the accumulation of carotenoids in this species. Remarkably, the two different branches of carotenoid synthesis were regulated depending on different light intensities. Contents of beta-carotene were 3-fold higher under low light intensities (33 mu mol m(-2) s(-1)) while lutein contents increased 1.5-fold under higher light intensities (170 and 280 mu mol m(-2) s(-1)). Nevertheless, highest contents of carotenoids (8.48 +/- 0.47 mg g(-1) DW) were found upon a thermal upshift from 20 degrees C to 35 degrees C after only two days at a light intensity of 170 mu mol m(-2) s(-1). Under these conditions, high contents of both lutein and beta-carotene were reached accounting for 3.17 +/- 0.18 and 3.21 +/- 0.18 mg g(-1) DW, respectively. This study indicates that Tetraselmis sp. CTP4 could be a sustainable source of lutein and beta-carotene at locations where a robust, euryhaline, thermotolerant microalgal strain is required.pt_PT
dc.description.sponsorshipPPBI-POCI-01-0145-FEDER-22122pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.1016/j.algal.2019.101732pt_PT
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.1/13522
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCentre of Marine Sciences
dc.relationBiomass value upgrade of a stress-resistant, lipid-rich marine microalga Tetraselmis sp. CTP4
dc.relationMicrobiome-mediated improvement of industrial Chlorella biomass production
dc.relationBiotechnological applications of a promising marine chlorophyte Tetraselmis sp. CTP4: a biorefinery approach
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMarine microalgaept_PT
dc.subjectCarotenoidspt_PT
dc.subjectShort-term stresspt_PT
dc.subjectRP-HPLCpt_PT
dc.subjectCarotenoid autofluorescencept_PT
dc.subjectNitrate repletionpt_PT
dc.titleImproved production of lutein and β-carotene by thermal and light intensity upshifts in the marine microalga Tetraselmis sp. CTP4pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleBiomass value upgrade of a stress-resistant, lipid-rich marine microalga Tetraselmis sp. CTP4
oaire.awardTitleMicrobiome-mediated improvement of industrial Chlorella biomass production
oaire.awardTitleBiotechnological applications of a promising marine chlorophyte Tetraselmis sp. CTP4: a biorefinery approach
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F115325%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F140143%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F105541%2F2014/PT
oaire.citation.startPage101732pt_PT
oaire.citation.titleAlgal Researchpt_PT
oaire.citation.volume45pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNameSchüler
person.familyNameSantos
person.familyNamePereira
person.familyNameKatkam
person.familyNameFlorindo
person.familyNameSchulze
person.familyNameBarreira
person.familyNameVarela
person.givenNameLisa
person.givenNameTamara
person.givenNameHugo
person.givenNameDr. Gangadhar
person.givenNameClaudia
person.givenNamePeter S.C.
person.givenNameLuísa
person.givenNameJoão
person.identifier282781
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person.identifier.ciencia-id6D1A-17E5-1400
person.identifier.orcid0000-0003-0961-5397
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person.identifier.orcid0000-0002-4077-855X
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridE-9360-2015
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person.identifier.scopus-author-id54080259900
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person.identifier.scopus-author-id56244247100
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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