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Increasing genomic information in bivalves through new EST collections in four species: Development of new genetic markers for environmental studies and genome evolution

2008Journal article Restricted
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dc.contributor.authorTanguy, A.
dc.contributor.authorBierne, N.
dc.contributor.authorSaavedra, Carlos
dc.contributor.authorPina, B.
dc.contributor.authorBachere, E.
dc.contributor.authorKube, M.
dc.contributor.authorBazin, E.
dc.contributor.authorBonhomme, F.
dc.contributor.authorBoudry, P.
dc.contributor.authorBoulo, V.
dc.contributor.authorBoutet, I.
dc.contributor.authorCancela, Leonor
dc.contributor.authorDossat, C.
dc.contributor.authorFavrel, P.
dc.contributor.authorHuvet, A.
dc.contributor.authorJarque, S.
dc.contributor.authorJollivet, D.
dc.contributor.authorKlages, Sven
dc.contributor.authorLapegue, S.
dc.contributor.authorLeite, Ricardo
dc.contributor.authorMoal, J.
dc.contributor.authorMoraga, D.
dc.contributor.authorReinhardt, Richard
dc.contributor.authorSamain, J. F.
dc.contributor.authorZouros, E.
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-13T08:53:37Z
dc.date.available2014-05-13T08:53:37Z
dc.date.issued2008
dc.date.updated2014-05-08T11:19:47Z
dc.description.abstractThe generation of EST information is an essential step in the genomic characterisation of species. In the context of the European Network Marine Genomics, a common goal was to significantly increase the amount of ESTs in commercial marine mollusk species and more specifically in the less studied but ecologically and commercially important groups, such as mussel and clam genera. Normalized cDNA libraries were constructed for four different relevant bivalves species (Crassostrea gigas, Mytilus edulis, Ruditapes decussatus and Bathymodiolus azoricus), using numerous tissues and physiological conditions. In this paper, we present the analysis of the 13,013 expressed sequence tags (ESTs) generated. Each EST library was independently assembled and 1300–3000 unique sequences were identified in each species. For the different species, functional categories could be assigned to only about 16 to 27% of ESTs using the GO annotation tool. All sequences have been incorporated into a publicly available database and form the basis for subsequent microarray design, SNP detection and polymorphism analysis, and the placement of novel markers on genetic linkage maps.por
dc.identifier.citationTanguy, A.; Bierne, N.; Saavedra, C.; Pina, B.; Bachere, E.; Kube, M.; Bazin, E.; Bonhomme, F.; Boudry, P.; Boulo, V.; Boutet, I.; Cancela, L.; Dossat, C.; Favrel, P.; Huvet, A.; Jarque, S.; Jollivet, D.; Klages, S.; Lapegue, S.; Leite, R.; Moal, J.; Moraga, D.; Reinhardt, R.; Samain, J. F.; Zouros, E.; Canario, A. V. M. Increasing genomic information in bivalves through new EST collections in four species: Development of new genetic markers for environmental studies and genome evolution, Gene, 408, 1-2, 27-36, 2008.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.gene.2007.10.021
dc.identifier.issn0378-1119
dc.identifier.otherAUT: LCA00739; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3879
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectMolluscspor
dc.subjectEvolutionpor
dc.subjectEnvironmentpor
dc.subjectESTspor
dc.titleIncreasing genomic information in bivalves through new EST collections in four species: Development of new genetic markers for environmental studies and genome evolutionpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage36por
oaire.citation.issue1-2por
oaire.citation.startPage27por
oaire.citation.titleGenepor
oaire.citation.volume408por
person.familyNameCancela
person.familyNameLeite
person.familyNameCanario
person.givenNameM. Leonor
person.givenNameRicardo
person.givenNameAdelino
person.identifier447622
person.identifier143624
person.identifier.ciencia-idF115-256E-C84D
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0002-9622-3895
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridB-3389-2008
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id8362650900
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication3fb360e0-a481-486e-96ca-af9f6ecd6eb8
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery3fb360e0-a481-486e-96ca-af9f6ecd6eb8

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