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Existence of a tightly regulated water channel in saccharomyces cerevisiae

2001-12Journal article Restricted access
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dc.contributor.authorMeyrial, V.
dc.contributor.authorLaizé, Vincent
dc.contributor.authorGobin, R.
dc.contributor.authorRipoche, P.
dc.contributor.authorHohmann, S.
dc.contributor.authorTacnet, F.
dc.date.accessioned2014-06-13T13:10:24Z
dc.date.available2014-06-13T13:10:24Z
dc.date.issued2001-12
dc.date.updated2014-06-03T10:24:41Z
dc.description.abstractThe Saccharomyces cerevisiae strain Σ1278b possesses two putative aquaporins, Aqy1-1p and Aqy2-1p. Previous work demonstrated that Aqy1-1p functions as a water channel in Xenopus oocyte. However, no function could be attributed to Aqy2-1p in this system. Specific antibodies were used to follow the expression of Aqy1-1p and Aqy2-1p in the yeast. Aqy1-1p was never detected whatever the growth phase and culture conditions tested. In contrast, Aqy2-1p was detected only during the exponential growth phase in rich medium containing glucose. Aqy2-1p expression was repressed by hyper-osmotic culture conditions. Both immunocytochemistry and biochemical subcellular fractionation demonstrated that Aqy2-1p is located on the endoplasmic reticulum (ER) as well as on the plasma membrane. In microsomal vesicles enriched in ER, a water channel activity due to Aqy2-1p was detected by stopped-flow analysis. Our results show that the expression of aquaporins is tightly controlled. The physiological relevance of aquaporin-mediated water transport in yeast is discussed.por
dc.identifier.citationMeyrial, V.; Laizé, V.; Gobin, R.; Ripoche, P.; Hohmann, S.; Tacnet, F.Existence of a tightly regulated water channel in saccharomyces cerevisiae, European Journal of Biochemistry, 268, 2, 334-343, 2001.por
dc.identifier.doihttp://doi:10.1152/ 10.1046/j.1432-1033.2001.01882.x
dc.identifier.issn0014-2956
dc.identifier.urihttp://hdl.handle.net/10400.1/4298
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1046/j.1432-1033.2001.01882.x/abstract;jsessionid=E7A2EF635FAC2FF1164638504E16C526.f03t02por
dc.subjectAquaporinpor
dc.subjectWater transportpor
dc.subjectSaccharomyces cerevisiaepor
dc.subjectAQY1por
dc.subjectAQY2por
dc.titleExistence of a tightly regulated water channel in saccharomyces cerevisiaepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage343por
oaire.citation.issue2por
oaire.citation.startPage334por
oaire.citation.titleEuropean Journal of Biochemistrypor
oaire.citation.volume268por
person.familyNameLaizé
person.givenNameVincent
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication.latestForDiscoveryb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f

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