Publication
A dual‐function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis
| dc.contributor.author | Carmo, Catarina | |
| dc.contributor.author | Coelho, João | |
| dc.contributor.author | Silva, Rui | |
| dc.contributor.author | Tavares, Alexandra | |
| dc.contributor.author | Boavida, Ana | |
| dc.contributor.author | Gaetani, Paola | |
| dc.contributor.author | Guilgur, Leonardo G | |
| dc.contributor.author | Martinho, Rui Goncalo | |
| dc.contributor.author | Oliveira, Raquel A | |
| dc.date.accessioned | 2023-09-13T10:41:18Z | |
| dc.date.available | 2023-09-13T10:41:18Z | |
| dc.date.issued | 2023 | |
| dc.description.abstract | Mitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs after substantial chromosome compaction and is not promoted by condensin I. Instead, we show that the timely removal of transcripts from mitotic chromatin is driven by the SNF2 helicase-like protein Lodestar (Lds), identified here as a modulator of sister chromatid cohesion defects. In addition to the eviction of nascent transcripts, we uncover that Lds cooperates with Topoisomerase 2 to ensure efficient sister chromatid resolution and mitotic fidelity. We conclude that the removal of nascent transcripts upon mitotic entry is not a passive consequence of cell cycle progression and/or chromosome compaction but occurs via dedicated mechanisms with functional parallelisms to sister chromatid resolution. | pt_PT |
| dc.description.sponsorship | DL57/2016/CP1361/CT0019 | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.15252/embr.202256463 | pt_PT |
| dc.identifier.issn | 1469-221X | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/19975 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Wiley | pt_PT |
| dc.relation | Chromosome-based mechanisms of transcriptional inactivation during mitosis | |
| dc.relation | Deciphering the minimal histone landscape required for mitotic chromosome architecture. | |
| dc.relation | Not Available | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Cohesin | pt_PT |
| dc.subject | Condensin | pt_PT |
| dc.subject | Mitotic transcription | pt_PT |
| dc.subject | SNF2 family | pt_PT |
| dc.subject | Topoisomerase 2 | pt_PT |
| dc.title | A dual‐function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Chromosome-based mechanisms of transcriptional inactivation during mitosis | |
| oaire.awardTitle | Deciphering the minimal histone landscape required for mitotic chromosome architecture. | |
| oaire.awardTitle | Not Available | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/H2020/101002391/EU | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//PD%2FBD%2F128428%2F2017/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F01092%2F2017%2FCP1424%2FCT0002/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/2022.01782.PTDC/PT | |
| oaire.citation.issue | 9 | pt_PT |
| oaire.citation.title | EMBO reports | pt_PT |
| oaire.citation.volume | 24 | pt_PT |
| oaire.fundingStream | H2020 | |
| oaire.fundingStream | CEEC IND 2017 | |
| oaire.fundingStream | 3599-PPCDT | |
| person.familyName | Silva | |
| person.familyName | Viegas Russo da Conceição Martinho | |
| person.givenName | Rui | |
| person.givenName | Rui Gonçalo | |
| person.identifier.ciencia-id | F31D-C783-3D89 | |
| person.identifier.orcid | 0000-0003-2168-8599 | |
| person.identifier.orcid | 0000-0002-1641-3403 | |
| person.identifier.rid | C-5622-2012 | |
| person.identifier.scopus-author-id | 15045741700 | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | European Commission | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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