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Impact of lead (Pb2+) on the growth and biological activity of Serratia marcescens selected for wastewater treatment and identification of its zntR gene—a metal efflux regulator

2023Journal article Restricted access
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dc.contributor.authordos Reis Ferreira, Gustavo Magno
dc.contributor.authorPires, Josiane Ferreira
dc.contributor.authorRibeiro, Luciana Silva
dc.contributor.authorDias Carlier, Jorge
dc.contributor.authorCosta, Maria Clara
dc.contributor.authorSchwan, Rosane Freitas
dc.contributor.authorSilva, Cristina Ferreira
dc.date.accessioned2023-06-26T13:46:17Z
dc.date.available2023-06-26T13:46:17Z
dc.date.issued2023
dc.description.abstractMicroorganisms isolated from contaminated areas play an important role in bioremediation processes. They promote heavy metal removal from the environment by adsorbing ions onto the cell wall surface, accumulating them inside the cells, or reducing, complexing, or precipitating these substances in the environment. Microorganism-based bioremediation processes can be highly efcient, low-cost and have low environmental impact. Thus, the present study aimed to select Pb2+-resistant bacteria and evaluate the growth rate, biological activity, and the presence of genes associated with metal resistance. Serratia marcescens CCMA 1010, that was previously isolated from cofee processing wastewater, was selected since was able to growth in Pb2+ concentrations of up to 4.0 mM. The growth rate and generation time did not difer from those of the control (without Pb2+), although biological activity decreased in the frst hour of exposure to these ions and stabilized after this period. The presence of the zntR, zntA and pbrA genes was analysed, and only zntR was detected. The zntR gene encodes a protein responsible for regulating the production of ZntA, a transmembrane protein that facilitates Pb2+ extrusion out of the cell. S. marcescens CCMA 1010 demonstrated a potential for use as bioindicator that has potential to be used in bioremediation processes due to its resistance to high concentrations of Pb2+, ability to grow until 24 h of exposure, and possession of a gene that indicates the existence of mechanisms associated with resistance to lead (Pb2+).pt_PT
dc.description.sponsorshipLA/P/0101/2020; CRESC Algarve 2020; project METALCHEMBIO (no. 29251)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s11274-023-03535-1pt_PT
dc.identifier.eissn1573-0972
dc.identifier.urihttp://hdl.handle.net/10400.1/19756
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.subjectBacteriapt_PT
dc.subjectBioremediationpt_PT
dc.subjectContamination metalspt_PT
dc.subjectLeadpt_PT
dc.subjectGene expression of metal resistancpt_PT
dc.titleImpact of lead (Pb2+) on the growth and biological activity of Serratia marcescens selected for wastewater treatment and identification of its zntR gene—a metal efflux regulatorpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.issue4pt_PT
oaire.citation.titleWorld Journal of Microbiology and Biotechnologypt_PT
oaire.citation.volume39pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCarlier
person.familyNameCosta
person.givenNameJorge
person.givenNameMaria Clara
person.identifier.ciencia-id691D-A1BB-15CC
person.identifier.ciencia-id7110-A28E-3C8A
person.identifier.orcid0000-0003-0675-2716
person.identifier.orcid0000-0003-1340-5237
person.identifier.ridM-6189-2013
person.identifier.scopus-author-id7102166949
person.identifier.scopus-author-id35354952400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication11c29f10-6e2b-40d8-9c74-2cc45be6d7a7
relation.isAuthorOfPublication.latestForDiscovery11c29f10-6e2b-40d8-9c74-2cc45be6d7a7
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication15f91d45-e070-47d8-b6b8-efd4de31d9a8
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