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Carbon partitioning and export in transgenic Arabidopsis thaliana with altered capacity for sucrose synthesis grown at low temperature: a role for metabolite transporters

2006-09Journal article Open access
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dc.contributor.authorLundmark, Maria
dc.contributor.authorCavaco, A. M.
dc.contributor.authorTrevanion, Stephen
dc.contributor.authorHurry, Vaughan
dc.date.accessioned2018-12-07T14:58:14Z
dc.date.available2018-12-07T14:58:14Z
dc.date.issued2006-09
dc.description.abstractWe investigated the role of metabolite transporters in cold acclimation by comparing the responses of wild-type (WT) Arabidopsis thaliana (Heynh.) with that of transgenic plants over-expressing sucrose-phosphate synthase (SPSox) or with that of antisense repression of cytosolic fructose-1,6-bisphosphatase (FBPas). Plants were grown at 23 degrees C and then shifted to 5 degrees C. We compared the leaves shifted to 5 degrees C for 3 and 10 d with new leaves that developed at 5 degrees C with control leaves on plants at 23 degrees C. At 23 degrees C, ectopic expression of SPS resulted in 30% more carbon being fixed per day and an increase in sucrose export from source leaves. This increase in fixation and export was supported by increased expression of the plastidic triose-phosphate transporter AtTPT and, to a lesser extent, the high-affinity Suc transporter AtSUC1. The improved photosynthetic performance of the SPSox plants was maintained after they were shifted to 5 degrees C and this was associated with further increases in AtSUC1 expression but with a strong repression of AtTPT mRNA abundance. Similar responses were shown by WT plants during acclimation to low temperature and this response was attenuated in the low sucrose producing FBPas plants. These data suggest that a key element in recovering flux through carbohydrate metabolism in the cold is to control the partitioning of metabolites between the chloroplast and the cytosol, and Arabidopsis modulates the expression of AtTPT to maintain balanced carbon flow. Arabidopsis also up-regulates the expression of AtSUC1, and to lesser extent AtSUC2, as down-stream components facilitate sucrose transport in leaves that develop at low temperatures.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1111/j.1365-3040.2006.01543.x
dc.identifier.issn0140-7791
dc.identifier.urihttp://hdl.handle.net/10400.1/11926
dc.language.isoeng
dc.peerreviewedyes
dc.publisherBlackwell Publishing
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTriose Phosphate/Phosphate Translocator
dc.subjectPhosphate Synthase Activity
dc.subjectPlants Nicotiana-Tabacum
dc.subjectAntisense Repression
dc.subjectFreezing Tolerance
dc.subjectBiosynthesis Pathway
dc.subjectCold-Acclimation
dc.subjectWinter Rye
dc.subjectFructose 2,6-Bisphosphate
dc.subjectStarch Degradation
dc.titleCarbon partitioning and export in transgenic Arabidopsis thaliana with altered capacity for sucrose synthesis grown at low temperature: a role for metabolite transporters
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1714
oaire.citation.issue9
oaire.citation.startPage1703
oaire.citation.titlePlant, Cell and Environment
oaire.citation.volume29
person.familyNameCavaco Guerra
person.givenNameAna Margarida
person.identifierC-1285-2012
person.identifier.ciencia-idC91E-B434-E327
person.identifier.orcid0000-0003-2708-5991
person.identifier.scopus-author-id6602899707
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication18ad736f-b1f3-4a19-9636-e7a80248cf94
relation.isAuthorOfPublication.latestForDiscovery18ad736f-b1f3-4a19-9636-e7a80248cf94

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