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STC1 interference on calcitonin family of receptors signaling during osteoblastogenesis via adenylate cyclase inhibition

2015-03Journal article Open access
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dc.contributor.authorTerra, Silvia R.
dc.contributor.authorCardoso, João CR
dc.contributor.authorFélix, Rute
dc.contributor.authorMartins, Leo Anderson M.
dc.contributor.authorSouza, Diogo Onofre G.
dc.contributor.authorGuma, Fatima C. R.
dc.contributor.authorCanario, Adelino
dc.contributor.authorSchein, Vanessa
dc.date.accessioned2018-12-07T14:53:40Z
dc.date.available2018-12-07T14:53:40Z
dc.date.issued2015-03
dc.description.abstractStanniocalcin 1 (STC1) and calcitonin gene-related peptide (CGRP) are involved in bone formation/remodeling. Here we investigate the effects of STC1 on functional heterodimer complex CALCRL/RAMP1, expression and activity during osteoblastogenesis. STC1 did not modify CALCRL and ramp 1 gene expression during osteoblastogenesis when compared to controls. However, plasma membrane spatial distribution of CALCRL/RAMP1 was modified in 7-day pre-osteoblasts exposed to either CGRP or STC1, and both peptides induced CALCRL and RAMP1 assembly. CGRP, but not STC1 stimulated cAMP accumulation in 7-day osteoblasts and in CALCRL/RAMP1 transfected HEK293 cells. Furthermore, STC1 inhibited forskolin stimulated cAMP accumulation of HEK293 cells, but not in CALCRL/RAMP1 transfected HEK293 cells. However, STC1 inhibited cAMP accumulation in calcitonin receptor (CTR) HEK293 transfected cells stimulated by calcitonin. In conclusion, STC1 signals through inhibitory G-protein modulates CGRP receptor spatial localization during osteoblastogenesis and may function as a regulatory factor interacting with calcitonin peptide members during bone formation. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
dc.description.sponsorshipCAPES/CNPq (VS PNPD fellowship program); FAPERGS/CNPq [008/2009 (FCRG)]
dc.identifier.doi10.1016/j.mce.2015.01.010
dc.identifier.issn0303-7207
dc.identifier.urihttp://hdl.handle.net/10400.1/11633
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Ireland
dc.subjectGene-Related Peptide
dc.subjectColocalization Analysis
dc.subjectStanniocalcin-1 Acts
dc.subjectBone Metabolism
dc.subjectTransgenic Mice
dc.subjectMessenger-Rnas
dc.subjectGrowth-Factor
dc.subjectIn-Vitro
dc.subjectCells
dc.subjectProtein
dc.titleSTC1 interference on calcitonin family of receptors signaling during osteoblastogenesis via adenylate cyclase inhibition
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F121279%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FMAR%2FLA0015%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F89811%2F2012/PT
oaire.citation.endPage87
oaire.citation.issueC
oaire.citation.startPage78
oaire.citation.titleMolecular and Cellular Endocrinology
oaire.citation.volume403
oaire.fundingStream3599-PPCDT
oaire.fundingStreamCOMPETE
oaire.fundingStreamSFRH
person.familyNameCardoso
person.familyNameFélix
person.familyNameCanario
person.familyNameSchein
person.givenNameJoão
person.givenNameRute
person.givenNameAdelino
person.givenNameVanessa
person.identifier14332
person.identifier143624
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-idFB1A-1325-8E25
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0002-4144-3452
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0001-7276-5772
person.identifier.ridM-4151-2013
person.identifier.ridM-6164-2013
person.identifier.ridC-7942-2009
person.identifier.ridA-3025-2014
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id6603316805
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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