Publication
STC1 interference on calcitonin family of receptors signaling during osteoblastogenesis via adenylate cyclase inhibition
dc.contributor.author | Terra, Silvia R. | |
dc.contributor.author | Cardoso, João CR | |
dc.contributor.author | Félix, Rute | |
dc.contributor.author | Martins, Leo Anderson M. | |
dc.contributor.author | Souza, Diogo Onofre G. | |
dc.contributor.author | Guma, Fatima C. R. | |
dc.contributor.author | Canario, Adelino | |
dc.contributor.author | Schein, Vanessa | |
dc.date.accessioned | 2018-12-07T14:53:40Z | |
dc.date.available | 2018-12-07T14:53:40Z | |
dc.date.issued | 2015-03 | |
dc.description.abstract | Stanniocalcin 1 (STC1) and calcitonin gene-related peptide (CGRP) are involved in bone formation/remodeling. Here we investigate the effects of STC1 on functional heterodimer complex CALCRL/RAMP1, expression and activity during osteoblastogenesis. STC1 did not modify CALCRL and ramp 1 gene expression during osteoblastogenesis when compared to controls. However, plasma membrane spatial distribution of CALCRL/RAMP1 was modified in 7-day pre-osteoblasts exposed to either CGRP or STC1, and both peptides induced CALCRL and RAMP1 assembly. CGRP, but not STC1 stimulated cAMP accumulation in 7-day osteoblasts and in CALCRL/RAMP1 transfected HEK293 cells. Furthermore, STC1 inhibited forskolin stimulated cAMP accumulation of HEK293 cells, but not in CALCRL/RAMP1 transfected HEK293 cells. However, STC1 inhibited cAMP accumulation in calcitonin receptor (CTR) HEK293 transfected cells stimulated by calcitonin. In conclusion, STC1 signals through inhibitory G-protein modulates CGRP receptor spatial localization during osteoblastogenesis and may function as a regulatory factor interacting with calcitonin peptide members during bone formation. (C) 2015 Elsevier Ireland Ltd. All rights reserved. | |
dc.description.sponsorship | CAPES/CNPq (VS PNPD fellowship program); FAPERGS/CNPq [008/2009 (FCRG)] | |
dc.identifier.doi | 10.1016/j.mce.2015.01.010 | |
dc.identifier.issn | 0303-7207 | |
dc.identifier.uri | http://hdl.handle.net/10400.1/11633 | |
dc.language.iso | eng | |
dc.peerreviewed | yes | |
dc.publisher | Elsevier Ireland | |
dc.subject | Gene-Related Peptide | |
dc.subject | Colocalization Analysis | |
dc.subject | Stanniocalcin-1 Acts | |
dc.subject | Bone Metabolism | |
dc.subject | Transgenic Mice | |
dc.subject | Messenger-Rnas | |
dc.subject | Growth-Factor | |
dc.subject | In-Vitro | |
dc.subject | Cells | |
dc.subject | Protein | |
dc.title | STC1 interference on calcitonin family of receptors signaling during osteoblastogenesis via adenylate cyclase inhibition | |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F121279%2F2010/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FMAR%2FLA0015%2F2013/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F89811%2F2012/PT | |
oaire.citation.endPage | 87 | |
oaire.citation.issue | C | |
oaire.citation.startPage | 78 | |
oaire.citation.title | Molecular and Cellular Endocrinology | |
oaire.citation.volume | 403 | |
oaire.fundingStream | 3599-PPCDT | |
oaire.fundingStream | COMPETE | |
oaire.fundingStream | SFRH | |
person.familyName | Cardoso | |
person.familyName | Félix | |
person.familyName | Canario | |
person.familyName | Schein | |
person.givenName | João | |
person.givenName | Rute | |
person.givenName | Adelino | |
person.givenName | Vanessa | |
person.identifier | 14332 | |
person.identifier | 143624 | |
person.identifier.ciencia-id | 8B16-F203-2AFC | |
person.identifier.ciencia-id | FB1A-1325-8E25 | |
person.identifier.ciencia-id | 1F1E-D3B3-F804 | |
person.identifier.orcid | 0000-0001-7890-0170 | |
person.identifier.orcid | 0000-0002-4144-3452 | |
person.identifier.orcid | 0000-0002-6244-6468 | |
person.identifier.orcid | 0000-0001-7276-5772 | |
person.identifier.rid | M-4151-2013 | |
person.identifier.rid | M-6164-2013 | |
person.identifier.rid | C-7942-2009 | |
person.identifier.rid | A-3025-2014 | |
person.identifier.scopus-author-id | 7201822956 | |
person.identifier.scopus-author-id | 56006773000 | |
person.identifier.scopus-author-id | 56568523700 | |
person.identifier.scopus-author-id | 6603316805 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
rcaap.rights | openAccess | |
rcaap.type | article | |
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relation.isProjectOfPublication.latestForDiscovery | b1e8418e-77aa-4452-a6c7-9aa3e86be4a2 |
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