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STC1 interference on calcitonin family of receptors signaling during osteoblastogenesis via adenylate cyclase inhibition

dc.contributor.authorTerra, Silvia R.
dc.contributor.authorCardoso, João CR
dc.contributor.authorFélix, Rute
dc.contributor.authorMartins, Leo Anderson M.
dc.contributor.authorSouza, Diogo Onofre G.
dc.contributor.authorGuma, Fatima C. R.
dc.contributor.authorCanario, Adelino
dc.contributor.authorSchein, Vanessa
dc.date.accessioned2018-12-07T14:53:40Z
dc.date.available2018-12-07T14:53:40Z
dc.date.issued2015-03
dc.description.abstractStanniocalcin 1 (STC1) and calcitonin gene-related peptide (CGRP) are involved in bone formation/remodeling. Here we investigate the effects of STC1 on functional heterodimer complex CALCRL/RAMP1, expression and activity during osteoblastogenesis. STC1 did not modify CALCRL and ramp 1 gene expression during osteoblastogenesis when compared to controls. However, plasma membrane spatial distribution of CALCRL/RAMP1 was modified in 7-day pre-osteoblasts exposed to either CGRP or STC1, and both peptides induced CALCRL and RAMP1 assembly. CGRP, but not STC1 stimulated cAMP accumulation in 7-day osteoblasts and in CALCRL/RAMP1 transfected HEK293 cells. Furthermore, STC1 inhibited forskolin stimulated cAMP accumulation of HEK293 cells, but not in CALCRL/RAMP1 transfected HEK293 cells. However, STC1 inhibited cAMP accumulation in calcitonin receptor (CTR) HEK293 transfected cells stimulated by calcitonin. In conclusion, STC1 signals through inhibitory G-protein modulates CGRP receptor spatial localization during osteoblastogenesis and may function as a regulatory factor interacting with calcitonin peptide members during bone formation. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
dc.description.sponsorshipCAPES/CNPq (VS PNPD fellowship program); FAPERGS/CNPq [008/2009 (FCRG)]
dc.identifier.doi10.1016/j.mce.2015.01.010
dc.identifier.issn0303-7207
dc.identifier.urihttp://hdl.handle.net/10400.1/11633
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Ireland
dc.subjectGene-Related Peptide
dc.subjectColocalization Analysis
dc.subjectStanniocalcin-1 Acts
dc.subjectBone Metabolism
dc.subjectTransgenic Mice
dc.subjectMessenger-Rnas
dc.subjectGrowth-Factor
dc.subjectIn-Vitro
dc.subjectCells
dc.subjectProtein
dc.titleSTC1 interference on calcitonin family of receptors signaling during osteoblastogenesis via adenylate cyclase inhibition
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F121279%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FMAR%2FLA0015%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F89811%2F2012/PT
oaire.citation.endPage87
oaire.citation.issueC
oaire.citation.startPage78
oaire.citation.titleMolecular and Cellular Endocrinology
oaire.citation.volume403
oaire.fundingStream3599-PPCDT
oaire.fundingStreamCOMPETE
oaire.fundingStreamSFRH
person.familyNameCardoso
person.familyNameFélix
person.familyNameCanario
person.familyNameSchein
person.givenNameJoão
person.givenNameRute
person.givenNameAdelino
person.givenNameVanessa
person.identifier14332
person.identifier143624
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-idFB1A-1325-8E25
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0002-4144-3452
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0001-7276-5772
person.identifier.ridM-4151-2013
person.identifier.ridM-6164-2013
person.identifier.ridC-7942-2009
person.identifier.ridA-3025-2014
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id6603316805
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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