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Multi-Omic profiling of macrophages treated with phospholipids containing Omega-3 and Omega-6 fatty acids reveals complex immunomodulatory adaptations at protein, lipid and metabolic levels

2022Journal article Open access
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dc.contributor.authorMaurício, Tatiana
dc.contributor.authorAveiro, Susana
dc.contributor.authorGuedes, Sofia
dc.contributor.authorLopes, Diana
dc.contributor.authorMelo, Tânia
dc.contributor.authorNeves, Bruno M.
dc.contributor.authorDomingues, Rosário
dc.contributor.authorDomingues, Pedro
dc.date.accessioned2022-07-13T10:48:56Z
dc.date.available2022-07-13T10:48:56Z
dc.date.issued2022
dc.description.abstractIn recent years, several studies have demonstrated that polyunsaturated fatty acids have strong immunomodulatory properties, altering several functions of macrophages. In the present work, we sought to provide a multi-omic approach combining the analysis of the lipidome, the proteome, and the metabolome of RAW 264.7 macrophages supplemented with phospholipids containing omega-3 (PC 18:0/22:6; omega 3-PC) or omega-6 (PC 18:0/20:4; omega 6-PC) fatty acids, alone and in the presence of lipopolysaccharide (LPS). Supplementation of macrophages with omega 3 and omega 6 phospholipids plus LPS produced a significant reprogramming of the proteome of macrophages and amplified the immune response; it also promoted the expression of anti-inflammatory proteins (e.g., pleckstrin). Supplementation with the omega 3-PC and omega 6-PC induced significant changes in the lipidome, with a marked increase in lipid species linked to the inflammatory response, attributed to several pro-inflammatory signalling pathways (e.g., LPCs) but also to the pro-resolving effect of inflammation (e.g., PIs). Finally, the metabolomic analysis demonstrated that supplementation with omega 3-PC and omega 6-PC induced the expression of several metabolites with a pronounced inflammatory and anti-inflammatory effect (e.g., succinate). Overall, our data show that supplementation of macrophages with omega 3-PC and omega 6-PC effectively modulates the lipidome, proteome, and metabolome of these immune cells, affecting several metabolic pathways involved in the immune response that are triggered by inflammation.pt_PT
dc.description.sponsorshipPOCI-01-0247-FEDER-035234, PPI/APM/2018/00015/U/001; LISBOA-01-0247-FEDER035234; ALG-01-0247-FEDER-035234; LISBOA-01-0145-FEDER-402-022125; CA19105
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms23042139pt_PT
dc.identifier.eissn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.1/17983
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMacrophagept_PT
dc.subjectOmega-6 phospholipidpt_PT
dc.subjectLPSpt_PT
dc.subjectProteomicspt_PT
dc.subjectLipidomicspt_PT
dc.subjectMetabolomicspt_PT
dc.titleMulti-Omic profiling of macrophages treated with phospholipids containing Omega-3 and Omega-6 fatty acids reveals complex immunomodulatory adaptations at protein, lipid and metabolic levelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.citation.issue4pt_PT
oaire.citation.startPage2139pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAveiro
person.givenNameSusana
person.identifier.ciencia-idD312-07B4-DA74
person.identifier.orcid0000-0002-6651-3226
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationed8b83e5-9fb0-4701-a65e-90690ccc139c
relation.isAuthorOfPublication.latestForDiscoveryed8b83e5-9fb0-4701-a65e-90690ccc139c
relation.isProjectOfPublicationb6f71df4-1d30-4f34-943f-48f234e0a9ef
relation.isProjectOfPublication010db565-8f84-4215-8139-a43c6816c7a0
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