Publication
The α5-α6-α7-Pba3-Pba4 complex: a starting unit in proteasome core particle assembly
| dc.contributor.author | Matias, Ana Catarina | |
| dc.contributor.author | Tiago, Maria Margarida | |
| dc.contributor.author | Zimmermann, Jessica | |
| dc.contributor.author | Dohmen, R. Jürgen | |
| dc.contributor.author | Ramos, Paula C. | |
| dc.date.accessioned | 2025-07-22T08:48:23Z | |
| dc.date.available | 2025-07-22T08:48:23Z | |
| dc.date.issued | 2025-05-08 | |
| dc.description.abstract | A complex composed of Pba3-Pba4 and subunits α5, α6, and α7 is identified as an early intermediate in proteasome core particle assembly in wild-type Saccharomyces cerevisiae cells. The same complex can be reconstituted from recombinantly produced components in vitro. Assembly of α6 and α7 with Pba3-Pba4 depends on the presence of the α5 subunit, the binding of which apparently initiates the formation of this intermediate. Our data suggest the following order of events: first, Pba3-Pba4 binds α5, then α6 is incorporated, and at the end α7. In the absence of the chaperones Pba1-Pba2 or Ump1, alternative Pba4-containing complexes are detected, the formation of which depends on the Blm10/PA200 protein. Overexpression of Pba1-Pba2 abolishes the formation of these complexes containing Pba4 and Blm10, suggesting that Blm10 may replace Pba1-Pba2 as an alternative assembly factor. | eng |
| dc.description.sponsorship | 442219341 | |
| dc.identifier.doi | 10.3390/biom15050683 | |
| dc.identifier.issn | 2218-273X | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/27473 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | MDPI | |
| dc.relation | Analysis of the early precursors in the 20S proteasome assembly pathway | |
| dc.relation | Control of eukaryotic proteasome biogenesis by chaperones | |
| dc.relation | Strategic Project - LA 23 - 2011-2012 | |
| dc.relation.ispartof | Biomolecules | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Proteasome core particle | |
| dc.subject | Assembly | |
| dc.subject | Chaperones | |
| dc.subject | Pba3-Pba4 | |
| dc.subject | Blm10 | |
| dc.title | The α5-α6-α7-Pba3-Pba4 complex: a starting unit in proteasome core particle assembly | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Analysis of the early precursors in the 20S proteasome assembly pathway | |
| oaire.awardTitle | Control of eukaryotic proteasome biogenesis by chaperones | |
| oaire.awardTitle | Strategic Project - LA 23 - 2011-2012 | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/POCI/POCI%2FBIA-PRO%2F58344%2F2004/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FQUI-BIQ%2F098427%2F2008/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0023%2F2011/PT | |
| oaire.citation.issue | 5 | |
| oaire.citation.title | Biomolecules | |
| oaire.citation.volume | 15 | |
| oaire.fundingStream | POCI | |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Matias | |
| person.familyName | Tiago | |
| person.familyName | Ramos | |
| person.givenName | Ana Catarina | |
| person.givenName | Maria Margarida | |
| person.givenName | Paula C. | |
| person.identifier.ciencia-id | 701D-019D-0779 | |
| person.identifier.ciencia-id | 811E-8F25-378A | |
| person.identifier.orcid | 0000-0002-6385-9117 | |
| person.identifier.orcid | 0009-0005-5007-3894 | |
| person.identifier.rid | A-9430-2014 | |
| person.identifier.scopus-author-id | 55232110200 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
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