Publication
Study of the antimicrobial and antioxidant effects of propolis extracts, a type of Algarve portuguese origin
| dc.contributor.advisor | Miguel, Maria Graça | |
| dc.contributor.advisor | Ferreira, Ana Luísa | |
| dc.contributor.author | Oliveira, Ana V. | |
| dc.date.accessioned | 2012-09-20T16:20:48Z | |
| dc.date.available | 2012-09-20T16:20:48Z | |
| dc.date.issued | 2009 | |
| dc.description | Dissertação de mest., Ciências Biomédicas, Departamento de Ciências Biomédicas e Medicina, Univ. do Algarve, 2009 | por |
| dc.description.abstract | In this work, extracts (aqueous, ethanolic and methanolic) of propolis harvested at two different times (winter and spring) from several locations of the Algarve region (B. N. Arrodeios, B. N. Pé da Serra, B. S. Arneijoafra and T. N. Madeira) were tested for their biological activities and composition. Results showed that propolis extracts were active against both Gram-positive and Gramnegative bacteria. All tested strains of bacteria showed susceptibility to the diluted propolis extracts (1:10) and in the majority of cases in a dose-dependent way. Most propolis samples collected at springtime showed higher antibacterial activity, in comparison with samples harvested at wintertime. There were also observed differences between collection sites and type of extract. These results correlate to HPLC results, where the same differences where observed. Regarding propolis citotoxicity, results showed that aqueous propolis extracts have no effect and ethanolic propolis extracts causes a small decrease in cell viability. Concerning antioxidant enzymatic activities, the superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (GP) activities of samples of propolis were determined. Results showed that SOD activity was dependent on the collection time and decreased drastically from winter to spring in samples from B.N. Arrodeios and B.S. Arneijoafra. The opposite was observed in samples from B.N. Pé da Serra and T.N. Madeira. We expected that the samples with higher SOD activities would have higher CAT activities also. Such was not observed, which may suggest that there could be other antioxidant enzymes involved, different to the ones tested. In respect with protein contents, major differences were not observed when comparing samples collected at different times, except for samples from B.S. Arneijoafra, where a decrease occurred from the sample collected at winter to sample collected at spring time. This was the first study of the biological activities of Portuguese propolis from the Algarve region. | por |
| dc.identifier.other | 615.3 OLI*Stu Cave | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/1660 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.subject | Própolis português | por |
| dc.subject | Actividade anti-bacteriana | por |
| dc.subject | Actividade antioxidante | por |
| dc.subject | Citotoxicidade | por |
| dc.subject | Composição química | por |
| dc.title | Study of the antimicrobial and antioxidant effects of propolis extracts, a type of Algarve portuguese origin | por |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | por |
| rcaap.type | masterThesis | por |
| thesis.degree.grantor | Universidade do Algarve. Departamento de Ciências Biomédicas e Medicina | por |
| thesis.degree.level | Mestre | por |
| thesis.degree.name | Mestrado em Ciências Biomédicas | por |