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Proximity ligation strategy for the genomic reconstruction of microbial communities associated with the ectoparasite Caligus rogercresseyi

2022-01Journal article Open Access
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dc.contributor.authorValenzuela-Miranda, Diego
dc.contributor.authorGonçalves, Ana Teresa
dc.contributor.authorValenzuela-Muñoz, Valentina
dc.contributor.authorNuñez-Acuña, Gustavo
dc.contributor.authorLiachko, Ivan
dc.contributor.authorNelson, Bradley
dc.contributor.authorGallardo-Escarate, Cristian
dc.date.accessioned2022-07-18T12:19:00Z
dc.date.available2022-07-18T12:19:00Z
dc.date.issued2022-01
dc.description.abstractThe sea louse Caligus rogercresseyi has become one of the main constraints for the sustainable development of salmon aquaculture in Chile. Although this parasite's negative impacts are well recognized by the industry, some novel potential threats remain unnoticed. The recent sequencing of the C. rogercresseyi genome revealed a large bacterial community associated with the sea louse, however, it is unknown if these microorganisms should become a new focus of sanitary concern. Herein, chromosome proximity ligation (Hi-C) coupled with long-read sequencing were used for the genomic reconstruction of the C. rogercresseyi microbiota. Through deconvolution analysis, we were able to assemble and characterize 413 bacterial genome clusters, including six bacterial genomes with more than 80% of completeness. The most represented bacterial genome belonged to the fish pathogen Tenacibacullum ovolyticum (97.87% completeness), followed by Dokdonia sp. (96.71% completeness). This completeness allowed identifying 21 virulence factors (VF) within the T. ovolyticum genome and four antibiotic resistance genes (ARG). Notably, genomic pathway reconstruction analysis suggests putative metabolic complementation mechanisms between C. rogercresseyi and its associated microbiota. Taken together, our data highlight the relevance of Hi-C techniques to discover pathogenic bacteria, VF, and ARGs and also suggest novel host-microbiota mutualism in sea lice biology.pt_PT
dc.description.sponsorshipFONDECYT FONDAP (#15110027), FONDECYT POSTDOC (#3200600), and FONDECYT (#1210852)
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-021-04485-0pt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/18020
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Researchpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLepeophtheirus-salmonispt_PT
dc.subjectResistance genespt_PT
dc.subjectTetracycline resistancept_PT
dc.subjectIntracellular bacteriapt_PT
dc.titleProximity ligation strategy for the genomic reconstruction of microbial communities associated with the ectoparasite Caligus rogercresseyipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage783pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume12pt_PT
person.familyNameFachadas Gato Coelho Gonçalves
person.givenNameAna Teresa
person.identifier.ciencia-id9F1F-939B-B01A
person.identifier.orcid0000-0002-5443-7182
person.identifier.scopus-author-id56308820700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication39c4122a-2552-4acd-b235-6083cb78f4f1
relation.isAuthorOfPublication.latestForDiscovery39c4122a-2552-4acd-b235-6083cb78f4f1

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