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Growth kinetics and physiological behavior of co-cultures of Saccharomyces cerevisiae and Kluyveromyces lactis, fermenting carob sugars extracted with whey

2016-10Journal article Restricted access
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dc.contributor.authorRodrigues, Brígida
dc.contributor.authorLima-Costa, Maria Emília
dc.contributor.authorConstantino, A.
dc.contributor.authorRaposo, Sara
dc.contributor.authorFelizardo, C.
dc.contributor.authorGonçalves, D.
dc.contributor.authorFernandes, T.
dc.contributor.authorDionísio, Lídia
dc.contributor.authorPeinado, J. M.
dc.date.accessioned2017-04-07T15:55:59Z
dc.date.available2017-04-07T15:55:59Z
dc.date.issued2016-10
dc.description.abstractAlcoholic fermentation of carob waste sugars (sucrose, glucose and fructose) extracted with cheese whey, by co-cultures of Saccharomyces.cerevisiae and Kluyveromyces lactis has been analyzed. Growth and fermentation of S. cerevisiae in the carob-whey medium showed an inhibition of about 30% in comparison with water-extracted carob. The inhibition of K. lactis on carob-whey was greater (70%) when compared with the whey medium alone, due to osmolarity problems. Oxygen availability was a very important factor for K. lactis, influencing its fermentation performance. When K. lactis was grown alone on carob-whey medium, lactose was always consumed first, and glucose and fructose were consumed afterwards, only at high aeration conditions. In co-culture with S. cerevisiae, K. lactis was completely inhibited and, at low aeration, died after 3 days; at high aeration this culture could survive but growth and lactose fermentation were only recovered after S. cerevisiae became stationary. To overcome the osmolarity and K. lactis' oxygen problems, the medium had to be diluted and a sequential fermentative process was designed in a STR-31 reactor. K. lactis was inoculated first and, with low aeration (0.13 vvm), consumed all the lactose in 48 h. Then S. cerevisiae was inoculated, consuming the total of the carob sugars, and producing ethanol in a fed-batch regime. The established co-culture with K. lactis increased S. cerevisiae ethanol tolerance. This fermentation process produced ethanol with good efficiency (80 g/l final concentration and a conversion factor of 0.4g ethanolig sugar), eliminating all the sugars of the mixed waste. These efficient fermentative results pointed to a new joint treatment of agro-industrial wastes which may be implemented successfully, with economic and environmental sustainability for a bioethanol industrial proposal. (C) 2016 Elsevier Inc. All rights reserved.
dc.identifier.doihttps://doi.org/10.1016/j.enzmictec.2016.06.012
dc.identifier.issn0141-0229
dc.identifier.urihttp://hdl.handle.net/10400.1/9279
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relation.isbasedonWOS:000382798000006
dc.subjectKluyveromyces lactis
dc.subjectSaccharomyces cerevisiae
dc.subjectCarob
dc.subjectWhey
dc.subjectWaste treatment
dc.subjectEthanol
dc.titleGrowth kinetics and physiological behavior of co-cultures of Saccharomyces cerevisiae and Kluyveromyces lactis, fermenting carob sugars extracted with whey
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-TEC%2F3346%2F2012/PT
oaire.citation.endPage48
oaire.citation.startPage41
oaire.citation.titleEnzyme and Microbial Technology
oaire.citation.volume92
oaire.fundingStream3599-PPCDT
person.familyNameRodrigues
person.familyNameLima Costa
person.familyNameRaposo
person.familyNameDionísio
person.givenNameBrígida
person.givenNameMaria Emília
person.givenNameSara
person.givenNameLídia
person.identifier.ciencia-id631C-A162-3F1E
person.identifier.ciencia-idF010-54F2-9E30
person.identifier.orcid0000-0001-9495-0813
person.identifier.orcid0000-0002-4433-5756
person.identifier.orcid0000-0001-5344-7349
person.identifier.orcid0000-0002-0530-9395
person.identifier.ridL-3673-2013
person.identifier.scopus-author-id7003633118
person.identifier.scopus-author-id7801639810
person.identifier.scopus-author-id6506947768
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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