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PTEN Mediates the Antioxidant Effect of Resveratrol at Nutritionally Relevant Concentrations

2014Journal article Open access
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dc.contributor.authorIngles, M.
dc.contributor.authorGambini, J.
dc.contributor.authorMiguel, Maria Graça
dc.contributor.authorBonet Costa, V.
dc.contributor.authorAbdelaziz, K. M.
dc.contributor.authorEl Alami, M.
dc.contributor.authorVina, J.
dc.contributor.authorBorras, C.
dc.date.accessioned2015-06-15T14:53:35Z
dc.date.available2015-06-15T14:53:35Z
dc.date.issued2014
dc.description.abstractIntroduction. Antioxidant properties of resveratrol have been intensively studied for the last years, both in vivo and in vitro. Its bioavailability after an oral dose is very low and therefore it is very important to make sure that plasma concentrations of free resveratrol are sufficient enough to be active as antioxidant. Aims. In the present study, using nutritionally relevant concentrations of resveratrol, we aim to confirm its antioxidant capacity on reducing peroxide levels and look for the molecular pathway involved in this antioxidant effect. Methods. We used mammary gland tumor cells (MCF-7), which were pretreated with different concentrations of resveratrol for 48 h, and/or a PTEN inhibitor (bpV: bipy). Hydrogen peroxide levels were determined by fluorimetry, PTEN levels and Akt phosphorylation by Western Blotting, and mRNA expression of antioxidant genes by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. Resveratrol treatment for 48 h lowered peroxide levels in MCF-7, even at low nutritional concentrations (1 nM). This effect was mediated by the activation of PTEN/Akt pathway, which resulted in an upregulation of catalase and MnSOD mRNA levels. Conclusion. Resveratrol acts as an antioxidant at nutritionally relevant concentrations by inducing the expression of antioxidant enzymes, through a mechanism involving PTEN/Akt signaling pathway.
dc.identifier.doihttps://dx.doi.org/10.1155/2014/580852
dc.identifier.issn2314-6133
dc.identifier.issn2314-6141
dc.identifier.otherAUT: MGM00322;
dc.identifier.urihttp://hdl.handle.net/10400.1/6347
dc.language.isoeng
dc.peerreviewedyes
dc.publisherHindawi Publishing Corporation
dc.relation.isbasedonP-009-B6V
dc.titlePTEN Mediates the Antioxidant Effect of Resveratrol at Nutritionally Relevant Concentrations
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage7
oaire.citation.startPage1
oaire.citation.titleBioMed Research International
oaire.citation.volume2014
person.familyNameMiguel
person.givenNameMaria da Graça
person.identifier.ciencia-id6110-6FE7-DF28
person.identifier.orcid0000-0003-2507-4228
person.identifier.scopus-author-id35562054500
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication2507350f-9e60-4d4a-a096-53e58d93549c
relation.isAuthorOfPublication.latestForDiscovery2507350f-9e60-4d4a-a096-53e58d93549c

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