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Independent effects of seawater pH and high P-CO2 on olfactory sensitivity in fish: possible role of carbonic anhydrase

2021-03Journal article Open access
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dc.contributor.authorVelez, Zélia
dc.contributor.authorCosta, Rita
dc.contributor.authorWang, Wenjing
dc.contributor.authorHubbard, Peter
dc.date.accessioned2021-09-08T10:58:03Z
dc.date.available2021-09-08T10:58:03Z
dc.date.issued2021-03
dc.description.abstractOcean acidificationmay alter olfactory-driven behaviour in fish by direct effects on the peripheral olfactory system; olfactory sensitivity is reduced in CO2-acidified seawater. The current study tested whether this is due to elevated P-CO2 or the consequent reduction in seawater pH and, if the former, the possible involvement of carbonic anhydrase, the enzyme responsible for the hydration of CO2 and production of carbonic acid. Olfactory sensitivity to amino acids was assessed by extracellularmulti-unit recording from the olfactory nerve of the gilthead seabream (Sparus aurata L.) in normal seawater (pH similar to 8.2), and after acute exposure to acidified seawater (pH similar to 7.7) but normal P-CO2 (similar to 340 mu atm) or to high P-CO2 seawater (similar to 1400 mu atm) at normal pH (similar to 8.2). Reduced pH in the absence of elevated P-CO2 caused a reduction in olfactory sensitivity to L-serine, L-leucine, L-arginine and L-glutamine, but not L-glutamic acid. Increased P-CO2 in the absence of changes in pH caused reduced olfactory sensitivity to L-serine, L-leucine and L-arginine, including increases in their threshold of detection, but had no effect on sensitivity to L-glutamine and L-glutamic acid. Inclusion of 1 mmol l(-1) acetazolamide (a membrane-permeant inhibitor of carbonic anhydrase) in the seawater reversed the inhibition of olfactory sensitivity to L-serine caused by high P-CO2. Ocean acidification may reduce olfactory sensitivity by reductions in seawater pH and intracellular pH (of olfactory receptor neurones); the former by reducing odorant-receptor affinity, and the latter by reducing the efficiency of olfactory transduction. The physiological role of carbonic anhydrase in the olfactory receptor neurones remains to be explored.
dc.description.sponsorshipFCT - Fundacao para a Ciencia e a TecnologiaPortuguese Foundation for Science and Technology [UIDB/04326/2020, PTDC/BIA-BMA/30262/2017, SFRH/BPD/100409/2014]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1242/jeb.238485
dc.identifier.issn0022-0949
dc.identifier.urihttp://hdl.handle.net/10400.1/17017
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCOMPANY BIOLOGISTS LTD
dc.relationAlgarve Centre for Marine Sciences
dc.relationHow ocean acidification affects olfaction in fish: a mechanistic approach.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectOcean acidification
dc.subjectOlfaction
dc.subjectAmino acid
dc.subjectOdorant-receptor affinity
dc.subjectOlfactory receptor
dc.subjectCarbon dioxide
dc.subject.otherLife Sciences & Biomedicine - Other Topics
dc.titleIndependent effects of seawater pH and high P-CO2 on olfactory sensitivity in fish: possible role of carbonic anhydrase
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleHow ocean acidification affects olfaction in fish: a mechanistic approach.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBIA-BMA%2F30262%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F100409%2F2014/PT
oaire.citation.issue6
oaire.citation.startPagejeb238485
oaire.citation.titleJournal of Experimental Biology
oaire.citation.volume224
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
person.familyNameVelez
person.familyNameCosta
person.familyNameWang
person.familyNameHubbard
person.givenNameZélia
person.givenNameRita
person.givenNameWenjing
person.givenNamePeter
person.identifier.ciencia-id4412-F95B-34A2
person.identifier.ciencia-id8219-DFF3-929F
person.identifier.ciencia-id951F-BA4B-A21F
person.identifier.orcid0000-0003-2761-4048
person.identifier.orcid0000-0002-6975-7576
person.identifier.orcid0000-0003-4836-1844
person.identifier.orcid0000-0002-3007-4647
person.identifier.ridM-5283-2013
person.identifier.ridO-9136-2018
person.identifier.ridB-2823-2008
person.identifier.scopus-author-id16176444400
person.identifier.scopus-author-id57097731700
person.identifier.scopus-author-id7005928467
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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