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Co-silencing of human Bub3 and dynein highlights an antagonistic relationship in regulating kinetochore-microtubule attachments

2015-11Journal article Open Access
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dc.contributor.authorSilva, Patricia M. A.
dc.contributor.authorTavares, Alvaro A.
dc.contributor.authorBousbaa, Hassan
dc.date.accessioned2018-12-07T14:57:55Z
dc.date.available2018-12-07T14:57:55Z
dc.date.issued2015-11
dc.description.abstractWe previously reported that the spindle assembly checkpoint protein Bub3 is involved in regulating kinetochore-microtubule (KT-MT) attachments. Also, Bub3 was reported to interact with the microtubule motor protein dynein. Here we examined how this interaction contributes to KT-MT attachments. Depletion of Bub3 or dynein induced misaligned chromosomes, consistent with their role in KT-MT attachments. Unexpectedly, co-silencing of both proteins partially suppressed the misalignment phenotype and restored chromosome congression. Consistent with these observations, KT-MT attachments in co-depleted cells were stable, able to drive chromosome congression, and produce inter-and intra-kinetochore stretch, indicating they are functional. We suggest that a mutual antagonism exists between Bub3 and dynein to ensure optimal KT-MT attachments. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
dc.description.sponsorshipCESPU [02-GCQF-CICS-2011N]; FCT - Fundacao para a Ciencia e a Tecnologia [CEQUIMED-PEst-OE/SAU/UI4040/2014]; FCT [SFRH/BD/90744/2012]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.febslet.2015.10.017
dc.identifier.issn0014-5793
dc.identifier.issn1873-3468
dc.identifier.urihttp://hdl.handle.net/10400.1/11770
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science Bv
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSpindle-assembly checkpoint
dc.subjectCytoplasmic dynein
dc.subjectMitotic checkpoint
dc.subjectMitosis
dc.subjectCongression
dc.subjectProtein
dc.subjectCells
dc.titleCo-silencing of human Bub3 and dynein highlights an antagonistic relationship in regulating kinetochore-microtubule attachments
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3594
oaire.citation.issue23
oaire.citation.startPage3588
oaire.citation.titleFEBS Letters
oaire.citation.volume589
person.familyNameSilva
person.familyNameTavares
person.givenNamePatrícia
person.givenNameAlvaro
person.identifier1445798
person.identifier.ciencia-id4B19-7E26-A047
person.identifier.ciencia-idAD12-B7F1-969B
person.identifier.orcid0000-0002-0694-7321
person.identifier.orcid0000-0002-7137-0944
person.identifier.scopus-author-id55855611900
person.identifier.scopus-author-id19036533500
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicationc2baf55d-94d7-4d71-b9f6-307d37cd4685
relation.isAuthorOfPublicationb44cba91-e2b1-4439-ba25-6ec1d68a059c
relation.isAuthorOfPublication.latestForDiscoveryc2baf55d-94d7-4d71-b9f6-307d37cd4685

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