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In vitro bioaccessibility of the marine biotoxins okadaic acid, dinophysistoxin-2 and their 7-O-acyl fatty acid ester derivatives in raw and steamed shellfish

2017-03Journal article Restricted access
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dc.contributor.authorManita, Diana
dc.contributor.authorAlves, Ricardo N.
dc.contributor.authorBraga, Ana Catarina
dc.contributor.authorFogaca, Fabiola H. S.
dc.contributor.authorMarques, Antonio
dc.contributor.authorReis Costa, Pedro
dc.date.accessioned2019-11-20T15:07:46Z
dc.date.available2019-11-20T15:07:46Z
dc.date.issued2017-03
dc.description.abstractOkadaic acid (OA), Dinophysistoxins (DTX1 and DTX2) and their acyl-derivatives (DTX3) are marine toxins responsible for the human diarrhetic shellfish poisoning. To date the amount of toxins ingested from consumption of shellfish has been considered equal to the amount of toxins available for uptake by the human body. The aim of this study is to assess the OA, DTX2 and DTX3 fractions released from raw and steamed mussels and cockles into the digestive fluids (bioaccessibility) using a static in vitro digestion model. Higher bioaccessibility was found in mussels (86 +/- 4%) than in cockles (59 +/- 9%). A significant reduction of ester derivatives of OA and an increase of OA were observed in the bioaccessible fraction of mussel samples, suggesting that DTX3 undergo conversion into their more toxic parent compounds during human digestion. However, similar increase of DTX2 and reduction of the respective acyl derivatives was not observed. Steaming lead to significant reduction of OA and analogues bioaccessibility in both species even though increased concentrations of toxins are obtained after this treatment. Risk assessment based solely on DSP toxins occurrence in seafood can conduct to an overestimation of the exposure and lead to more conservative regulatory measures. (C) 2017 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipEuropean Community [311820]
dc.description.sponsorshipTOXMASS (Operational Fisheries Program - Promar)
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT) [UID/Multi/04326/2013]
dc.description.sponsorshipPortuguese Science and Technology Foundation (FCT) [PD/BD/113484/2015]
dc.description.sponsorshipFCT Investigator program (IF)
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.fct.2017.01.608
dc.identifier.issn0278-6915
dc.identifier.issn1873-6351
dc.identifier.urihttp://hdl.handle.net/10400.1/13210
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPergamon-Elsevier Science
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectChromatography-mass-spectrometry
dc.subjectProtein phosphatases
dc.subjectPoisoning toxins
dc.subjectDsp toxins
dc.subjectDigestion
dc.subjectMussels
dc.subjectHydrolysis
dc.subjectToxicity
dc.subjectNorway
dc.subjectCoast
dc.titleIn vitro bioaccessibility of the marine biotoxins okadaic acid, dinophysistoxin-2 and their 7-O-acyl fatty acid ester derivatives in raw and steamed shellfish
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.endPage127
oaire.citation.startPage121
oaire.citation.titleFood and Chemical Toxicology
oaire.citation.volume101
oaire.fundingStream5876
person.familyNameReis Costa
person.givenNamePedro
person.identifier600820
person.identifier.ciencia-idC911-9715-E547
person.identifier.orcid0000-0001-6083-470X
person.identifier.ridN-1908-2019
person.identifier.scopus-author-id7201895802
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication24ad7119-d61f-4bdb-a5d5-9bd6e2c37312
relation.isAuthorOfPublication.latestForDiscovery24ad7119-d61f-4bdb-a5d5-9bd6e2c37312
relation.isProjectOfPublication868b4818-3efa-4edb-9202-c464d64fd38f
relation.isProjectOfPublication.latestForDiscovery868b4818-3efa-4edb-9202-c464d64fd38f

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