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Development and characterization of highly polymorphic microsatellite loci for the Western Spadefoot toad, Pelobates cultripes

2009Journal article Restricted access
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dc.contributor.authorVan De Vliet, M. S.
dc.contributor.authorDiekmann, O. E.
dc.contributor.authorSerrão, Ester
dc.contributor.authorBeja, Pedro
dc.date.accessioned2014-06-03T13:30:59Z
dc.date.available2014-06-03T13:30:59Z
dc.date.issued2009
dc.date.updated2014-05-21T13:19:50Z
dc.description.abstractNine highly polymorphic microsatellite markers were isolated and characterized for the Western Spadefoot, Pelobates cultripes. Remarkably, for this amphibian species high numbers of microsatellites were found as part of larger repeat containing regions, making primer design difficult. For nine loci, primers were designed successfully and genotyping of individuals was reliable and consistent. Number of alleles and heterozygosity for these loci ranged from 9 to 34 and from 0.72 to 0.94, respectively. The high levels of polymorphism revealed by our developed loci should provide insight into population genetic structure and levels of dispersal for this typical Mediterranean temporary pond-breeding amphibian.por
dc.identifier.citationVan De Vliet, M.S.; Diekmann, O.E.; Serrão, E.T.A.; Beja, P. Development and characterization of highly polymorphic microsatellite loci for the Western Spadefoot toad, Pelobates cultripes, Conservation Genetics, 10, 4, 993-996, 2009.por
dc.identifier.doihttp://dx.doi.org/10.1007/s10592-008-9670-6
dc.identifier.issn1566-0621
dc.identifier.otherAUT: ESE00527;
dc.identifier.urihttp://hdl.handle.net/10400.1/4163
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.subjectPelobates cultripespor
dc.subjectWestern Spadefootpor
dc.subjectPelobates fuscuspor
dc.subjectMicrosatellitespor
dc.subjectCross-species amplificationpor
dc.subjectTemporary pondspor
dc.subjectAmphibian conservation geneticspor
dc.titleDevelopment and characterization of highly polymorphic microsatellite loci for the Western Spadefoot toad, Pelobates cultripespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage996por
oaire.citation.issue4por
oaire.citation.startPage993por
oaire.citation.titleConservation Geneticspor
oaire.citation.volume10por
person.familyNameSerrao
person.familyNameCorreia de Oliveira Beja
person.givenNameEster A.
person.givenNamePedro Rui
person.identifierC-6686-2012
person.identifier6xecf34AAAAJ
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.ciencia-idC116-3331-F9E6
person.identifier.orcid0000-0003-1316-658X
person.identifier.orcid0000-0001-8164-0760
person.identifier.scopus-author-id7004093604
person.identifier.scopus-author-id6602807125
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication45ccfe90-155c-4d6f-9e86-8f0fd064005f
relation.isAuthorOfPublication74f734df-0ea1-43de-9ed7-d0c731ab7c07
relation.isAuthorOfPublication.latestForDiscovery45ccfe90-155c-4d6f-9e86-8f0fd064005f

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