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Novel galanin receptors in teleost fish: identification, expression and regulation by sex steroids

2014-09-01Journal article Open access
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dc.contributor.authorMartins, Rute S. T.
dc.contributor.authorPinto, Patricia IS
dc.contributor.authorGuerreiro, P. M.
dc.contributor.authorZanuy, Silvia
dc.contributor.authorCarrillo, Manuel
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-12-12T11:53:54Z
dc.date.available2014-12-12T11:53:54Z
dc.date.issued2014-09-01
dc.description.abstractIn fish, the onset of puberty, the transition from juvenile to sexually reproductive adult animals, is triggered by the activation of pituitary gonadotrophin secretion and its timing is influenced by external and internal factors that include the growth/adiposity status of the animal. Kisseptins have been implicated in the activation of puberty but peripheral signals coming from the immature gonad or associated to the metabolic/nutritional status are also thought to be involved. Additionally, there is evidence that the galaninergic system in the brain and testis of pre-pubertal male sea bass is a possible mediator involved in the translation of somatic signals leading to gonadal maturation. Here, the transcripts for four galanin receptors (GALR), named GALR1a, 1b, 2a and 2b, were isolated from European sea bass, Dicentrarchus labrax. Phylogenetic analysis confirmed the previously reported duplication of GALR1 in teleost fish, and unravelled the duplication of GALR2 in teleost fish and in some tetrapod species. Comparison with human showed that the key amino acids involved in ligand binding are present in the corresponding GALR1 and GALR2 orthologues. Transcripts for all four receptors are expressed in brain and testes of adult fish with GALR1a and GALR1b abundant in testes 15 and hardly detected in ovaries. In order to investigate whether GALR1 dimorphic expression was dependent on steroid context we evaluated the effect of 11-ketotestosterone and 17β-estradiol treatments on the receptor expression in brain and testes of pre-pubertal males. Interestingly, steroid treatments had no effect on the expression of GALRs in the brain while in the testes, GALR1a and GALR1b were significantly up regulated by 11KT. Altogether, these results support a role for the galaninergic system, in particular the GALR1 paralog in fish reproductive function.por
dc.identifier.issn0016-6480
dc.identifier.otherAUT: PMG01122; ACA00258
dc.identifier.urihttp://hdl.handle.net/10400.1/5680
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
dc.relation.publisherversionhttp://ac.els-cdn.com/S0016648014002639/1-s2.0-S0016648014002639-main.pdf?_tid=5580f8ca-812c-11e4-8bb2-00000aacb35f&acdnat=1418298994_1593eaa0e2140c5a3a32416c36a02796por
dc.subjectGalanin receptorspor
dc.subjectPubertypor
dc.subjectSea basspor
dc.subjectEstradiolpor
dc.subject11-Ketotestosteronepor
dc.titleNovel galanin receptors in teleost fish: identification, expression and regulation by sex steroidspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/222719/EU
oaire.citation.endPage120por
oaire.citation.startPage109por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume205por
oaire.fundingStreamFP7
person.familyNamePinto
person.familyNameGuerreiro da Costa Guerreiro
person.familyNameCanario
person.givenNamePatricia IS
person.givenNamePedro Miguel
person.givenNameAdelino
person.identifier643457
person.identifierA-2539-2009
person.identifier143624
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-3817-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id56568523700
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublicationd0a8a148-98e7-4899-8fb0-0b8afa91e4a4
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isProjectOfPublicationeb95e695-02d8-41f1-88df-951429f0f4f3
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