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Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification

2017-08Journal article Open access
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dc.contributor.authorRuocco, Miriam
dc.contributor.authorMusacchia, Francesco
dc.contributor.authorOlivé, Irene
dc.contributor.authorCosta, Monya
dc.contributor.authorBarrote, Isabel
dc.contributor.authorSantos, Rui
dc.contributor.authorSanges, Remo
dc.contributor.authorProcaccini, Gabriele
dc.contributor.authorSilva, João
dc.date.accessioned2019-11-20T15:07:28Z
dc.date.available2019-11-20T15:07:28Z
dc.date.issued2017-08
dc.description.abstractHere, we report the first use of massive-scale RNA-sequencing to explore seagrass response to CO2-driven ocean acidification (OA). Large-scale gene expression changes in the seagrass Cymodocea nodosa occurred at CO2 levels projected by the end of the century. C. nodosa transcriptome was obtained using Illumina RNA-Seq technology and de novo assembly, and differential gene expression was explored in plants exposed to short-term high CO2/low pH conditions. At high pCO(2), there was a significant increased expression of transcripts associated with photosynthesis, including light reaction functions and CO2 fixation, and also to respiratory pathways, specifically for enzymes involved in glycolysis, in the tricarboxylic acid cycle and in the energy metabolism of the mitochondrial electron transport. The upregulation of respiratory metabolism is probably supported by the increased availability of photo-synthates and increased energy demand for biosynthesis and stress-related processes under elevated CO2 and low pH. The upregulation of several chaperones resembling heat stress-induced changes in gene expression highlighted the positive role these proteins play in tolerance to intracellular acid stress in seagrasses. OA further modifies C. nodosa secondary metabolism inducing the transcription of enzymes related to biosynthesis of carbon-based secondary compounds, in particular the synthesis of polyphenols and isoprenoid compounds that have a variety of biological functions including plant defence. By demonstrating which physiological processes are most sensitive to OA, this research provides a major advance in the understanding of seagrass metabolism in the context of altered seawater chemistry from global climate change.
dc.description.sponsorshipPortuguese FCT project HighGrass [PTDC/MAR-EST/3687/2012]
dc.identifier.doi10.1111/mec.14204
dc.identifier.eissn1365-294X
dc.identifier.issn0962-1083
dc.identifier.urihttp://hdl.handle.net/10400.1/13055
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationHigh-CO2 effects on seagrass photosynthetic ecophysiology
dc.subjectElevated atmospheric Co2
dc.subjectCarbon nutrient balance
dc.subjectGene-expression
dc.subjectClimate change
dc.subjectPosidonia-Oceanica
dc.subjectZostera-Marina
dc.subjectSaccharomyces-Cerevisiae
dc.subjectSeawater acidification
dc.subjectHerbivore performance
dc.subjectCalcifying organisms
dc.subjectCarbohydrate metabolism
dc.subjectCymodocea nodosa
dc.subjectOcean acidification
dc.subjectProtein folding
dc.subjectSeagrasses
dc.subjectTranscriptome
dc.titleGenomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHigh-CO2 effects on seagrass photosynthetic ecophysiology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-EST%2F3687%2F2012/PT
oaire.citation.endPage4259
oaire.citation.issue16
oaire.citation.startPage4241
oaire.citation.titleMolecular Ecology
oaire.citation.volume26
oaire.fundingStream3599-PPCDT
person.familyNameRuocco
person.familyNameOlivé
person.familyNameCosta
person.familyNameBarrote
person.familyNameSantos
person.familyNameSilva
person.givenNameMiriam
person.givenNameIrene
person.givenNameMonya
person.givenNameIsabel
person.givenNameRui
person.givenNameJoão
person.identifier42560
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person.identifier.orcid0000-0002-4865-923X
person.identifier.orcid0000-0002-4236-9063
person.identifier.orcid0000-0002-0922-0259
person.identifier.orcid0000-0002-7861-4366
person.identifier.orcid0000-0002-7211-1661
person.identifier.ridJ-3922-2013
person.identifier.ridB-4168-2008
person.identifier.ridJ-7874-2013
person.identifier.scopus-author-id16242114500
person.identifier.scopus-author-id8094380800
person.identifier.scopus-author-id8659654200
person.identifier.scopus-author-id7201375018
person.identifier.scopus-author-id7403023547
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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