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A yeast assay based on the gilthead sea bream (teleost fish) estrogen receptor ß for monitoring estrogen mimics

2009Journal article Restricted access
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dc.contributor.authorPassos, A. L.
dc.contributor.authorPinto, Patricia IS
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-14T10:46:44Z
dc.date.available2014-05-14T10:46:44Z
dc.date.issued2009
dc.date.updated2014-05-08T10:39:14Z
dc.description.abstractA yeast(Saccharomyces cerevisiae)-based assay was developed and tested with steroids and chemicals (mostly pesticides). The induction of b-galactosidase activity was strictly dependent on the presence of seabream (Sparus aurata) ba estrogen receptor(sbERba) and substances known to have estrogenic activity. 17b-Estradiol (E2) and diethylstilbestrol(DES), both agonists, were most active and the antagonist tamoxifen(TAM)was14-fold less active than E2. Among the chemicals tested bisphenol-A was most active, followed by pentachlorophenol and naphthalene. Ligand-binding assays with recombinant sbERba and sbERa revealed that sbERba binds E2 with 6.5-fold higher affinity than sbERa, confirming the selection of a high sensitive receptor for the yeast assay. DES, ICI182, 780,estrone and TAM had higher relative binding affinity to E2 in sbERa than sbERba, although there was no difference in IC50 for these steroids between the two receptors. These results reveal the use fulness of using they east-based receptor assay for detecting chemical interaction with steroid receptors from contaminated samples.por
dc.identifier.citationPassos, Ana L.S.; Pinto, Patrícia I.S.; Power, Deborah M.; Canario, Adelino V.M. A yeast assay based on the gilthead sea bream (teleost fish) estrogen receptor ß for monitoring estrogen mimics, Ecotoxicology and Environmental Safety, 72, 5, 1529-1537, 2009.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ecoenv.2009.02.004
dc.identifier.issn0147-6513
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3928
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectEndocrine disrupterspor
dc.subjectPesticidespor
dc.subjectYeast assaypor
dc.subjectEstrogen receptorpor
dc.titleA yeast assay based on the gilthead sea bream (teleost fish) estrogen receptor ß for monitoring estrogen mimicspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1537por
oaire.citation.issue5por
oaire.citation.startPage1529por
oaire.citation.titleEcotoxicology and Environmental Safetypor
oaire.citation.volume72por
person.familyNamePinto
person.familyNamePower
person.familyNameCanario
person.givenNamePatricia IS
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier643457
person.identifier143624
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-3817-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

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