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Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations

2001-09Journal article Open access
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dc.contributor.authorCancela, M. Leonor
dc.contributor.authorOhresser, M. C. P.
dc.contributor.authorReia, J. P.
dc.contributor.authorS B Viegas, Carla
dc.contributor.authorWilliamson, M. K.
dc.contributor.authorPrice, P. A.
dc.date.accessioned2018-12-07T14:58:15Z
dc.date.available2018-12-07T14:58:15Z
dc.date.issued2001-09
dc.description.abstractMatrix Gla protein (MGP) belongs to the family of vitamin K-dependent, Gla-containing proteins and in higher vertebrates, is found in the extracellular matrix of mineralized tissues and soft tissues. MGP synthesis is highly regulated at the transcription and posttranscription levels and is now known to be involved in the regulation of extracellular matrix calcification and maintenance of cartilage and soft tissue integrity during growth and development. However, its mode of action at the molecular level remains unknown. Because there is a large degree of conservation between amino,acid sequences of shark and human MGP, the function of MGP probably has been conserved throughout evolution. Given the complexity of the mammalian system, the study of MGP in a lower vertebrate might be advantageous to relate the onset of MGP expression with specific events during development. Toward this goal, MGP was purified from Xenopus long bones and its N-terminal amino acid sequence was determined and used to clone the Xenopus MGP complementary DNA (cDNA) by a mixture of reverse-transcription (RT)- and 5'- rapid amplification of cDNA ends (RACE)-polymerase chain reaction (PCR). MGP messenger RNA (mRNA) was present in all tissues analyzed although predominantly expressed in Xenopus bone and heart and its presence was detected early in development at the onset of chondrocranium development and long before the appearance of the first calcified structures and metamorphosis. These results show that in this system, as in mammals, MGP may be required to delay or prevent mineralization of cartilage and soft tissues during the early stages of development and indicate that Xenopus is an adequate model organism to further study MGP function during growth and development.
dc.description.sponsorshipNATO/CRG940751/SA5.2.05, Praxis XXI/BIA 469/94, (NIH; grant AR 25921) (Praxis XXI/BPD/18816) (Praxis XXI/BICJ-2985)
dc.identifier.doi10.1359/jbmr.2001.16.9.1611
dc.identifier.issn0884-0431
dc.identifier.urihttp://hdl.handle.net/10400.1/11930
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.subjectCarboxyglutamic acid protein
dc.subjectSubstrate recognition
dc.subjectMessenger-rna
dc.subjectBone
dc.subjectRat
dc.subjectSequence
dc.subjectExpression
dc.subjectGene
dc.subjectIdentification
dc.subjectCartilage
dc.subjectMatrix Gla protein
dc.subjectXenopus laevis
dc.subjectcDNA
dc.subjectEvolution
dc.subjectDevelopment
dc.titleMatrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1621
oaire.citation.issue9
oaire.citation.startPage1611
oaire.citation.titleJournal of Bone and Mineral Research
oaire.citation.volume16
person.familyNameCancela
person.familyNameViegas
person.givenNameM. Leonor
person.givenNameCarla
person.identifier.ciencia-idC414-F596-EEC6
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0002-5765-3665
person.identifier.ridN-6695-2014
person.identifier.scopus-author-id8656310300
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication33984b3c-ffac-477a-896a-ddbf7aced4e3
relation.isAuthorOfPublication.latestForDiscoveryb9bbfe32-3dfe-4131-ad14-a4394008447f

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