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Lab-scale optimization of aurantiochytrium sp. culture medium for improved growth and DHA production

2020-04-05Artigo científico Acesso aberto
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datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorTrovão, Mafalda
dc.contributor.authorPereira, Hugo
dc.contributor.authorCosta, Margarida
dc.contributor.authorMachado, Adriana
dc.contributor.authorBarros, Ana
dc.contributor.authorSoares, Maria
dc.contributor.authorCarvalho, Bernardo
dc.contributor.authorSilva, Joana Teles
dc.contributor.authorVarela, João
dc.contributor.authorSilva, Joana
dc.date.accessioned2026-02-27T13:20:45Z
dc.date.available2026-02-27T13:20:45Z
dc.date.issued2020-04-05
dc.description.abstractThraustochytrids have gained increasing relevance over the last decades, due to their fast growth and outstanding capacity to accumulate polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA). In this context, the present work aimed to optimize the growth performance and DHA yields by improving the culture medium of Aurantiochytrium sp. AF0043. Accordingly, two distinct culture media were optimized: (i) an inorganic optimized medium (IOM), containing only monosodium glutamate and glucose as nitrogen and carbon sources, respectively; and (ii) an organic and sustainable waste-based optimized medium (WOM), containing corn steep powder and glycerol, added in fed-batch mode, as nitrogen and carbon sources, respectively. Overall, the lab-scale optimization allowed to increase the biomass yield 1.5-fold and enhance DHA content 1.7-fold using IOM. Moreover, WOM enabled a 2-fold increase in biomass yield and a significant improvement in lipid contents, from 22.78% to 31.14%. However, DHA content was enhanced almost 3-fold, from an initial content of 10.12% to 29.66% of total fatty acids contained in the biomass. Therefore, these results strongly suggest, not only that the production pipeline was significantly improved but also confirmed the potential use of Aurantiochytrium sp. AF0043 as a source of DHA.eng
dc.description.sponsorshipPOCI-01-0247-FEDER-035234; LISBOA-01-0247-FEDER-035234; ALG-01-0247-FEDER-035234
dc.identifier.doi10.3390/app10072500
dc.identifier.issn2076-3417
dc.identifier.urihttp://hdl.handle.net/10400.1/28283
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationAlgarve Centre for Marine Sciences
dc.relation.ispartofApplied Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectThraustochytrids
dc.subjectAurantiochytrium sp.
dc.subjectDocosahexaenoic acid (DHA)
dc.subjectCulture medium optimization
dc.subjectGrowth performance
dc.titleLab-scale optimization of aurantiochytrium sp. culture medium for improved growth and DHA productioneng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.citation.issue7
oaire.citation.startPage2500
oaire.citation.titleApplied sciences
oaire.citation.volume10
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePereira
person.familyNameVarela
person.givenNameHugo
person.givenNameJoão
person.identifier282781
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridE-9360-2015
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id54080259900
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublicationebcb2180-5235-4502-b562-cd1e7786e6f9
relation.isAuthorOfPublicationd339a9e7-bc32-49c4-ba48-a17343d40092
relation.isAuthorOfPublication.latestForDiscoveryebcb2180-5235-4502-b562-cd1e7786e6f9
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication.latestForDiscoveryfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f

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