Repository logo
 
Publication

Insights from bacteroides species in children with Type 1 Diabetes

2021-07-02Journal article Open access
Simple item page
dc.contributor.authorMatos, José
dc.contributor.authorMatos, Isabel
dc.contributor.authorCalha, Maria
dc.contributor.authorSantos, Pedro
dc.contributor.authorDuarte, Isabel
dc.contributor.authorCardoso, Yameric
dc.contributor.authorFaleiro, Maria Leonor
dc.date.accessioned2021-07-28T12:54:52Z
dc.date.available2021-07-28T12:54:52Z
dc.date.issued2021-07-02
dc.date.updated2021-07-23T13:26:50Z
dc.description.abstract: In our previous study the enrichment of the intestinal proteome of type 1 diabetes (T1D) children with Bacteroides proteins was observed, which led us to our current study that aimed to isolate and characterize Bacteroides species from fecal samples of T1D and control children. Repetitive sequence-based PCR (rep-PCR) was used for typing the isolated Bacteroides species. The antibiotic susceptibility and mucinolytic activity of the isolates was determined. The quantification of specific bacterial groups in the fecal samples was determined by qPCR. The ability to adhere and invade the human colonic cell line HT29-MTX-E12 of strains of P. dorei, B. uniformis and P. distasonis was determined and their whole genome sequencing was performed. The results showed similar numbers of Bacteroides species in T1D and control samples, but unique Bacteroides species and a higher recovery of P. distasonis from T1D samples was observed. Rep-PCR grouped the different Bacteroides species, but no discrimination by origin was achieved. T1D children showed a significant increase in Proteobacteria and a depletion in Lactobacillus sp. All tested P. dorei, B. uniformis and P. distasonis were able to adhere to HT29-MTX-E12 cells but significant differences (p < 0.05) in the ability to invade was observed. The highest ability to invade was exhibited by P. distasonis PtF D14MH1 and P. dorei PtFD16P1, while B. uniformis strains were unable to invade. The damage to tight junctions was also observed. The presence of Lactobacillus sp. inhibited the invasion ability of P. distasonis PtF D14MH1 but not P. dorei PtFD16P1. Sequences of agonist peptides of the human natural preproinsulin and the insulin B chain insB:9-23 peptide mimics were identified. The results reported in our study stresses the continued efforts required to clarify the link between T1D and gut microbiota.pt_PT
dc.description.sponsorshipPPBI-POCI-01-0145-FEDER-22122pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms 9 (7): 1436 (2021)pt_PT
dc.identifier.doi10.3390/microorganisms9071436pt_PT
dc.identifier.eissn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.1/16829
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectType 1 diabetespt_PT
dc.subjectPhocaeicola doreipt_PT
dc.subjectParabacteroides distasonispt_PT
dc.subjectBacteroides uniformispt_PT
dc.subjectInvasionpt_PT
dc.subjectPhagept_PT
dc.subjectMolecular mimicrypt_PT
dc.titleInsights from bacteroides species in children with Type 1 Diabetespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue7pt_PT
oaire.citation.startPage1436pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume9pt_PT
person.familyNameSoares Curado de Matos
person.familyNameFélix Calha
person.familyNameSantos
person.familyNameCardoso
person.familyNameFaleiro
person.givenNameIsabel Cristina
person.givenNameMaria Manuela
person.givenNamePedro
person.givenNameYameric
person.givenNameMaria Leonor
person.identifier252666
person.identifier.ciencia-idFF1D-581F-7EF1
person.identifier.ciencia-id3012-9A19-5CB2
person.identifier.ciencia-id281D-5E01-F09A
person.identifier.orcid0000-0002-1470-6181
person.identifier.orcid0000-0002-4550-6350
person.identifier.orcid0000-0001-8166-1596
person.identifier.orcid0000-0002-3878-6948
person.identifier.scopus-author-id6507927018
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationef87f069-b271-4c09-98b2-9058dd891d5f
relation.isAuthorOfPublication4f5a57d9-4969-4b52-b3e5-b46f945de649
relation.isAuthorOfPublication493e0b49-b458-42fc-8ae1-7ba4147ca950
relation.isAuthorOfPublication99f436b1-ba10-4d3f-813f-c18b77bad4ab
relation.isAuthorOfPublication5361e29a-3b79-4ce8-9f87-0c667d60588d
relation.isAuthorOfPublication.latestForDiscovery5361e29a-3b79-4ce8-9f87-0c667d60588d

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
microorganisms-09-01436-v2.pdf
Size:
4.31 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FCT2-Artigos (em revistas ou actas indexadas)
FCB2-Artigos (em revistas ou actas indexadas)
ULS2-Artigos (em revistas ou actas indexadas)