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Transcriptional and cellular effects of paracetamol in the oyster Crassostrea gigas

2017-10Journal article Restricted
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dc.contributor.authorBebianno, Maria João
dc.contributor.authorMello, A. C. P.
dc.contributor.authorSerrano, M. A. S.
dc.contributor.authorFlores-Nunes, F.
dc.contributor.authorMattos, J. J.
dc.contributor.authorZacchi, F. L.
dc.contributor.authorPiazza, C. E.
dc.contributor.authorSiebert, M. N.
dc.contributor.authorPiazza, R. S.
dc.contributor.authorGomes, C. H. A. M.
dc.contributor.authorMelo, C. M. R.
dc.contributor.authorBainy, A. C. D.
dc.date.accessioned2019-11-20T15:07:20Z
dc.date.available2019-11-20T15:07:20Z
dc.date.issued2017-10
dc.description.abstractAcetaminophen (paracetamol) (PAR) is one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) with analgesic and antipyretic properties consumed worldwide and often detected in the aquatic environment. Due to the fact that PAR induces oxidative stress in mammals, the aim of this study was to evaluate if similar effects were observed in oysters Crassostrea gigas, given their economic and ecological importance and world-wide distribution. Oysters were exposed for 1, 4 and 7 days to two different sublethal PAR concentrations (0, 1 and 100 mu g L-1). Cell viability, DNA damage in hemocytes and enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH) and glutathione S-transferases (GST) were evaluated in oyster gills. In addition, changes at transcriptional level of Cu/Zn superoxide distnutase (SOD), catalase-like (CAT-like), cytochrome P450 genes (CYP30C1, CYP2AU2, CYP3071A1, CYP356A1), glutathione S-transferase isoforms (GST-omega and GST-pi-like), cyclooxygenase (COX), fatty acid binding proteins-like (FABP-like), and caspase genes were evaluated in oyster gills and digestive gland. No changes in cell viability and DNA damage were observed in oysters exposed to both PAR concentrations. Similarly, no significant changes were detected in the major antioxidant enzymes (except for auxiliary enzyme GR) in oyster gills, suggesting that changes in GR activity are enough to counteract a potential oxidative stress in C. gigas gills under these experimental conditions. Furthermore, changes at transcriptional level are concentration and tissue dependent. PAR elicited an inhibition of CYP30C1, CYP3071A1 and FABP-like transcripts highlighting their role in drug metabolism, transport and detoxification of PAR in the gills. GST transcript levels were type, tissue and concentration-dependent. GST-pi-like was down-regulated in oyster gills exposed to the lowest PAR concentration and up-regulated in the digestive gland of oysters exposed to the highest PAR concentration. However, GST-omega transcript levels were lower only in oysters digestive gland exposed to the lowest PAR concentration. Therefore, changes at transcriptional level were more sensitive to assess the exposure to PAR at environmental relevant concentrations.
dc.description.sponsorshipproject UNIVERSAL - MCTI/CNPq [14/2012 (483028/21012-6)]
dc.description.sponsorshipMSC grant PEC-PG/CNPq
dc.description.sponsorshipCNPq [307467/2013-9]
dc.description.sponsorshipPVE-CNPq [406104/2013-1]
dc.identifier.doi10.1016/j.ecoenv.2017.06.034
dc.identifier.issn0147-6513
dc.identifier.issn1090-2414
dc.identifier.urihttp://hdl.handle.net/10400.1/12985
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAcademic Press Inc Elsevier Science
dc.subjectAntiinflammatory drugs nsaids
dc.subjectEnvironmental risk-assessment
dc.subjectClam ruditapes-philippinarum
dc.subjectWaste-water
dc.subjectMytilus-galloprovincialis
dc.subjectPharmaceutical compounds
dc.subjectDreissena-polymorpha
dc.subjectCorbicula-fluminea
dc.subjectBiomarker approach
dc.subjectMarine organisms
dc.titleTranscriptional and cellular effects of paracetamol in the oyster Crassostrea gigas
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage267
oaire.citation.startPage258
oaire.citation.titleEcotoxicology and Environmental Safety
oaire.citation.volume144
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32

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