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Isolation and characterization of a stress-inducible Dunaliella salina Lcy-β gene encoding a functional lycopene β-cyclase

2008Journal article Restricted access
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dc.contributor.authorRamos, Ana
dc.contributor.authorCoesel, Sacha
dc.contributor.authorMarques, Ana
dc.contributor.authorRodrigues, Marta
dc.contributor.authorBaumgartner, Alexandra
dc.contributor.authorNoronha, João
dc.contributor.authorRauter, Amélia
dc.contributor.authorBrenig, Bertram
dc.contributor.authorVarela, J.
dc.date.accessioned2014-07-24T09:26:00Z
dc.date.available2014-07-24T09:26:00Z
dc.date.issued2008
dc.description.abstractThe halotolerant green alga Dunaliella salina accumulates large amounts of β-carotene when exposed to various stress conditions. Although several studies concerning accumulation and biotechnological production of β-carotene have been published, the molecular basis and regulation of the genes involved in carotenoid biosynthesis in D. salina are still poorly known. In this paper, we report the isolation and regulation of the lycopene β-cyclase (Lcy-β) gene by abiotic stress. The function of this gene was determined by heterologous genetic complementation in E. coli. Gene expression and physiological analyses revealed that D. salina Lcy-β steady-state transcript and carotenoid levels were up-regulated in response to all stress conditions tested (salt, light and nutrient depletion). The results presented here suggest that nutrient availability is a key factor influencing carotenogenesis as well as carotenoid biosynthesis-related gene expression in D. salina.por
dc.description.sponsorshipFCT, European Unionpor
dc.description.sponsorshipOVERCAROTEN POCTI/MAR/15237/99; INTERREG 159-SAL—Atlantic Salt Ponds
dc.identifier.citationRamos, A., S. Coesel, A. Marques, M. Rodrigues, A. Baumgartner, J. Noronha, A. Rauter, B. Brenig & J. Varela (2008). Isolation and characterization of a stress-inducible Dunaliella salina Lcy-β gene encoding a functional lycopene beta-cyclase. Applied Microbiology and Biotechnology, 79: 819-828.por
dc.identifier.doihttp://dx.doi.org/10.1007/s00253-008-1492-4
dc.identifier.otherAUT: JVA01305;
dc.identifier.urihttp://hdl.handle.net/10400.1/4838
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relationMOLECULAR BASIS OF ISOPRENOID BIOSYNTHESIS IN DUNALIELLA SALINA
dc.relationREGULATION OF THE CAROTENOID BIOSYNTHETIC PATHWAY IN THE HALOTOLERANT MICROALGA DUNALIELLA SALINA
dc.subjectCarotenoid biosynthesispor
dc.subjectDunaliella salinapor
dc.subjectLycopene β-cyclasepor
dc.subjectGene expressionpor
dc.subjectStress response regulationpor
dc.titleIsolation and characterization of a stress-inducible Dunaliella salina Lcy-β gene encoding a functional lycopene β-cyclasepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMOLECULAR BASIS OF ISOPRENOID BIOSYNTHESIS IN DUNALIELLA SALINA
oaire.awardTitleREGULATION OF THE CAROTENOID BIOSYNTHETIC PATHWAY IN THE HALOTOLERANT MICROALGA DUNALIELLA SALINA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F13937%2F2003/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F4839%2F2001/PT
oaire.citation.endPage828por
oaire.citation.startPage819por
oaire.citation.titleApplied Microbiology and Biotechnologypor
oaire.citation.volume79por
person.familyNameRamos
person.familyNameVarela
person.givenNameAna
person.givenNameJoão
person.identifier.ciencia-id761B-F84D-0CF5
person.identifier.orcid0000-0003-1099-4005
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridM-3815-2013
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication42936018-1328-4fd1-8adb-4d37441727ab
relation.isAuthorOfPublicationd339a9e7-bc32-49c4-ba48-a17343d40092
relation.isAuthorOfPublication.latestForDiscovery42936018-1328-4fd1-8adb-4d37441727ab
relation.isProjectOfPublication87f3d167-a603-4907-9364-be92fab44fda
relation.isProjectOfPublicationdb83d956-ee85-4be6-8700-95d124d87cc3
relation.isProjectOfPublication.latestForDiscovery87f3d167-a603-4907-9364-be92fab44fda

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