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Advisor(s)
Abstract(s)
Vine shoots are the main by-products of grapevine pruning with no added value. In the present study, deep
eutectic solvents (DESs) were used as alternatives to traditional chemical solvents, for the extraction of phyto chemicals from grapevine shoots. Three levulinic acid-based DESs were tested for the first time, and their per formance was compared to methanol (a standard chemical solvent) regarding the extraction of phenolic
compounds from thirteen Vitis sp. shoots. Two extraction methods have been applied: ultrasound-assisted
extraction and solid-liquid extraction. A total of eleven polyphenols which belongs to four families (proantho cyanins, stilbenes, hydroxycinnamic acids, and flavonols) have been identified and quantified in the extracts.
The statistical analysis shows that the levulinic acid-based DES systems are novel and important alternatives to
chemical solvents due to favourable eco-friendly properties and remarkable extraction performance of poly phenols. On the other hand, the ultrasound-assisted extraction technique has significantly increased the
extraction rate in comparison to the solid-liquid extraction method with p-values lower than 0.05 for most
compounds. The genetic factor has been shown to play an important role in the content of extracted polyphenols,
being V. riparia pubescente the one that presented the highest concentrations of extracted polyphenols. Finally,
the polyphenol-enriched extracts have proven important properties such as antioxidant activity and significant
delay in bacteria growth against both gram-positive and gram-negative bacteria. It is important to note that, to
the best of our knowledge, this is the first time that deep eutectic solvents have been used for the extraction of
bioactive compounds from vine shoot residues.
Description
Keywords
Grapevine shoots Vitis sp Polyphenol extraction Deep eutectic solvents Antioxidant properties Circular economy
Citation
Publisher
Elsevier