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Gender-specific histopathological response in guppies Poecilia reticulata exposed to glyphosate or its metabolite aminomethylphosphonic acid

2017-09Journal article Restricted access
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dc.contributor.authorAntunes, Adriana Maria
dc.contributor.authorLopes Rocha, Thiago
dc.contributor.authorPires, Fernando Santiago
dc.contributor.authorde Freitas, Meire Alves
dc.contributor.authorMilhomem Cruz Leite, Vanessa Rafaela
dc.contributor.authorArana, Sarah
dc.contributor.authorMoreira, Paulo Cesar
dc.contributor.authorTeixeira Saboia-Morais, Simone Maria
dc.date.accessioned2019-11-20T15:07:24Z
dc.date.available2019-11-20T15:07:24Z
dc.date.issued2017-09
dc.description.abstractEcotoxicity of glyphosate (GLY) and itsmetabolite aminomethylphosphonic acid (AMPA) was investigated in guppies, Poecilia reticulata. We tested the effects of these chemicals on the gills and liver of bothmale and female guppies using qualitative and quantitative histopathological analyses associated with histopathological condition indexes. Both genders showed similar median lethal concentration (LC50) at 96 h for GLY (68.78 and 70.87mg l(-1)) and AMPA (180 and 164.32mg l(-1)). However, the histopathological assessment of both fish organs exposed to sublethal concentrations of GLY (35mg l(-1)) and AMPA ( 82mg l(-1)) for 96h showed a tissue-and gender-specific histopathological response. In both exposure assays, fish presented mainly progressive changes, such as proliferation of the interlamellar epithelium, partial and total fusion of secondary lamellae. The liver showed mainly regressive changes, such as steatosis, pyknotic nuclei and high distribution of collagen fibers. Unusually large hepatocytes as degenerated cells were also detected. Histopathological changes in gills were similar for the males and females, but the liver response was different between the genders. The hepatic inflammatory changes were more common in males. The increase in the area of hepatocyte vacuoles is gender dependent with higher values in the male compared to the female guppies exposed to GLY and AMPA. Multiparametric analysis indicated that the male guppies are-more sensitive than females, particularly in the presence of AMPA. Our study shows that the histopathological assessment associated with gender-specific response can be successfully used in ecotoxicological assessment of GLY and the metabolite AMPA. Copyright (C) 2017 JohnWiley & Sons, Ltd.
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [038-PROCAD NF 2009-2013]
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq) [473572/2010-9]
dc.identifier.doi10.1002/jat.3461
dc.identifier.issn0260-437X
dc.identifier.issn1099-1263
dc.identifier.urihttp://hdl.handle.net/10400.1/13023
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.subjectOxidative stress
dc.subjectMicrocystin-Lr
dc.subjectDna-damage
dc.subjectFish
dc.subjectHerbicide
dc.subjectLiver
dc.subjectGills
dc.subjectTissues
dc.subjectZebrafish
dc.subjectToxicity
dc.titleGender-specific histopathological response in guppies Poecilia reticulata exposed to glyphosate or its metabolite aminomethylphosphonic acid
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1107
oaire.citation.issue9
oaire.citation.startPage1098
oaire.citation.titleJournal of Applied Toxicology
oaire.citation.volume37
person.familyNameLopes Rocha
person.givenNameThiago
person.identifier.orcid0000-0003-0551-6842
person.identifier.ridA-3864-2015
person.identifier.scopus-author-id37007842200
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicationce186883-7988-4912-a5b7-39ce48201c27
relation.isAuthorOfPublication.latestForDiscoveryce186883-7988-4912-a5b7-39ce48201c27

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