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Skin damage caused by scale loss modifies the intestine of chronically stressed gilthead sea bream (Sparus aurata, L.)

2020-12Journal article Restricted access
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dc.contributor.authorMateus, Ana
dc.contributor.authorMourad, Mona
dc.contributor.authorPower, Deborah
dc.date.accessioned2021-01-12T10:48:46Z
dc.date.available2021-12-01T01:30:15Z
dc.date.issued2020-12
dc.description.abstractThe present study was designed to test if the damage caused by scale loss provokes a change in other innate immune barriers such as the intestine and how chronic stress affects this response. Sea bream (Sparus aurata) were kept in tanks at low density (16 kg m−3, LD) or exposed to a chronic high density (45 kg m−3, HD) stress for 4 weeks. Scales were then removed (approximately 50%) from the left flank in the LD and HD fish. Intestine samples (n = 8/group) were examined before and at 12 h, 3 days and 7 days after scale removal. Changes in the morphology of the intestine revealed that chronic stress and scale loss was associated with intestinal inflammation. Specifically, enterocyte height and the width of the lamina propria, submucosa and muscle layer were significantly increased (p < 0.05) 3 days after skin damage in fish under chronic stress (HD) compared to other treatments (LDWgut3d or HDgut0h). This was associated with a significant up-regulation (p < 0.05) in the intestine of gene transcripts for cell proliferation (pcna) and anti-inflammatory cytokine tgfβ1 and down-regulation of gene transcripts for the pro-inflammatory cytokines tnf-α and il1β (p < 0.05) in HD and LD fish 3 days after scale removal compared to the undamaged control (LDgut0h). Furthermore, a significant up-regulation of kit, a marker of mast cells, in the intestine of HDWgut3d and LDWgut3d fish suggests they may mediate the crosstalk between immune barriers. Skin damage induced an increase in cortisol levels in the anterior intestine in HDWgut12 h fish and significant (p < 0.05) down-regulation of mr expression, irrespective of stress. These results suggest glucocorticoid levels and signalling in the intestine of fish are modified by superficial cutaneous wounds and it likely modulates intestine inflammation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.dci.2020.103989pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/14951
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectCutaneous woundpt_PT
dc.subjectInnate immune barrierpt_PT
dc.subjectIntestinept_PT
dc.subjectMast cellspt_PT
dc.subjectMelanomacrophagept_PT
dc.subjectMucosal barrierpt_PT
dc.subjectCortisolpt_PT
dc.titleSkin damage caused by scale loss modifies the intestine of chronically stressed gilthead sea bream (Sparus aurata, L.)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage103989pt_PT
oaire.citation.titleDevelopmental & Comparative Immunologypt_PT
oaire.citation.volume118pt_PT
person.familyNameMateus
person.familyNamePower
person.givenNameAna Patrícia
person.givenNameDeborah Mary
person.identifier.ciencia-id2816-E389-626E
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-2045-1047
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication74cac9a6-f4f8-438b-a60e-a2b4d3312102
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery74cac9a6-f4f8-438b-a60e-a2b4d3312102

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