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Specific evolution and gene family expansion of complement 3 and regulatory factor H in fish

2020Journal article Open access
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dc.contributor.authorNajafpour, Babak
dc.contributor.authorCardoso, João CR
dc.contributor.authorCanario, Adelino
dc.contributor.authorPower, Deborah
dc.date.accessioned2021-01-14T20:23:00Z
dc.date.available2021-01-14T20:23:00Z
dc.date.issued2020
dc.description.abstractThe complement system comprises a large family of plasma proteins that play a central role in innate and adaptive immunity. To better understand the evolution of the complement system in vertebrates and the contribution of complement to fish immunity comprehensive in silico and expression analysis of the gene repertoire was made. Particular attention was given to C3 and the evolutionary related proteins C4 and C5 and to one of the main regulatory factors of C3b, factor H (Cfh). Phylogenetic and gene linkage analysis confirmed the standing hypothesis that the ancestral c3/c4/c5 gene duplicated early. The duplication of C3 (C3.1 and C3.2) and C4 (C4.1 and C4.2) was likely a consequence of the (1R and 2R) genome tetraploidization events at the origin of the vertebrates. In fish, gene number was not conserved and multiple c3 and cfh sequence related genes were encountered, and phylogenetic analysis of each gene generated two main clusters. Duplication of c3 and cfh genes occurred across the teleosts in a species-specific manner. In common, with other immune gene families the c3 gene expansion in fish emerged through a process of tandem gene duplication. Gilthead sea bream (Sparus aurata), had nine c3 gene transcripts highly expressed in liver although as reported in other fish, extra-hepatic expression also occurs. Differences in the sequence and protein domains of the nine deduced C3 proteins in the gilthead sea bream and the presence of specific cysteine and N-glycosylation residues within each isoform was indicative of functional diversity associated with structure. The diversity of C3 and other complement proteins as well as Cfh in teleosts suggests they may have an enhanced capacity to activate complement through direct interaction of C3 isoforms with pathogenic agents.pt_PT
dc.description.sponsorship(FCT) project to CCMAR (UIDB/04326/2020)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3389/fimmu.2020.568631pt_PT
dc.identifier.issn1664-3224
dc.identifier.urihttp://hdl.handle.net/10400.1/14966
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.relationConsumer driven Production: Integrating Innovative Approaches for Competitive and Sustainable Performance across the Mediterranean Aquaculture Value Chain
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectComplement systempt_PT
dc.subjectEnvironmentpt_PT
dc.subjectSkinpt_PT
dc.subjectLiverpt_PT
dc.subjectEvolutionpt_PT
dc.subjectFishpt_PT
dc.subjectInnate immunitypt_PT
dc.titleSpecific evolution and gene family expansion of complement 3 and regulatory factor H in fishpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleConsumer driven Production: Integrating Innovative Approaches for Competitive and Sustainable Performance across the Mediterranean Aquaculture Value Chain
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/727610/EU
oaire.citation.startPage568631pt_PT
oaire.citation.titleFrontiers in Immunologypt_PT
oaire.citation.volume11pt_PT
oaire.fundingStreamH2020
person.familyNameNAJAFPOUR
person.familyNameCardoso
person.familyNameCanario
person.familyNamePower
person.givenNameBABAK
person.givenNameJoão
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifier14332
person.identifier143624
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-2977-482X
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-4151-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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