Repository logo
 
Publication

Metabolic rate and rates of protein turnover in food-deprived cuttlefish, Sepia officinalis (Linnaeus 1758)

2016-06Journal article Open access
Simple item page
dc.contributor.authorLamarre, Simon G.
dc.contributor.authorMacCormack, Tyson J.
dc.contributor.authorSykes, António
dc.contributor.authorHall, Jennifer R.
dc.contributor.authorSpeers-Roesch, Ben
dc.contributor.authorCallaghan, Neal I.
dc.contributor.authorDriedzic, William R.
dc.date.accessioned2017-04-07T15:56:36Z
dc.date.available2017-04-07T15:56:36Z
dc.date.issued2016-06
dc.description.abstractTo determine the metabolic response to food deprivation, cuttlefish (Sepia officinalis) juveniles were either fed, fasted (3 to 5 days food deprivation), or starved (12 days food deprivation). Fasting resulted in a decrease in triglyceride levels in the digestive gland, and after 12 days, these lipid reserves were essentially depleted. Oxygen consumption was decreased to 53% and NH4 excretion to 36% of the fed group following 3-5 days of food deprivation. Oxygen consumption remained low in the starved group, but NH4 excretion returned to the level recorded for fed animals during starvation. The fractional rate of protein synthesis of fasting animals decreased to 25% in both mantle and gill compared with fed animals and remained low in the mantle with the onset of starvation. In gill, however, protein synthesis rate increased to a level that was 45% of the fed group during starvation. In mantle, starvation led to an increase in cathepsin A-, B-, H-, and L-like enzyme activity and a 2.3-fold increase in polyubiquitin mRNA that suggested an increase in ubiquitin-proteasome activity. In gill, there was a transient increase in the polyubiquitin transcript levels in the transition from fed through fasted to the starved state and cathepsin A-, B-, H-, and L-like activity was lower in starved compared with fed animals. The response in gill appears more complex, as they better maintain rates of protein synthesis and show no evidence of enhanced protein breakdown through recognized catabolic processes.
dc.description.sponsorshipSEPIATECH 31-03-05-FEP-2 Program PROMAR
dc.identifier.doi10.1152/ajpregu.00459.2015
dc.identifier.issn0363-6119
dc.identifier.urihttp://hdl.handle.net/10400.1/9467
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Physiological Society
dc.relationTowards cephalopod aquaculture: cuttlefish as the spearhead species
dc.relation.isbasedonWOS:000377021700017
dc.subjectNH4 production
dc.subjectAmmonia quotient
dc.subjectCathepsin
dc.titleMetabolic rate and rates of protein turnover in food-deprived cuttlefish, Sepia officinalis (Linnaeus 1758)
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTowards cephalopod aquaculture: cuttlefish as the spearhead species
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00576%2F2014%2FCP1217%2FCT0002/PT
oaire.citation.endPageR1168
oaire.citation.issue11
oaire.citation.startPageR1160
oaire.citation.titleAJP - Regulatory, Integrative and Comparative Physiology
oaire.citation.volume310
oaire.fundingStreamInvestigador FCT
person.familyNameSykes
person.givenNameAntónio
person.identifier107454
person.identifier.ciencia-id7510-6641-5A42
person.identifier.orcid0000-0002-5207-0612
person.identifier.ridC-3609-2012
person.identifier.scopus-author-id7102626426
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicationb9d55f95-5de8-40cf-82a3-96ec98c91a55
relation.isAuthorOfPublication.latestForDiscoveryb9d55f95-5de8-40cf-82a3-96ec98c91a55
relation.isProjectOfPublicationa7bbb7aa-912c-4d7f-933a-3905800d8dcf
relation.isProjectOfPublication.latestForDiscoverya7bbb7aa-912c-4d7f-933a-3905800d8dcf

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
9467.pdf
Size:
170.78 KB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)