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Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa
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Orientador(es)
Resumo(s)
In order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the seagrass Cymodocea nodosa,
microsatellite markers were obtained by screening a genomic library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified polymorphic microsatellites and are described. These loci identified a number of alleles ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76, when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we describe here the multiplexing conditions for 12 of these loci.
Descrição
Palavras-chave
Clonal plant Cymodocea nodosa Microsatellites Seagrass
Contexto Educativo
Citação
Alberto, F.; Correia, L.; Billot, C.; Duarte, C.M.; Serrão, E.A.Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa, Molecular Ecology Notes, 3, 3, 397-399, 2003.
Editora
Wiley-Blackwell