Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6249
Título: East Adriatic: a reservoir region of severe Citrus tristeza virus strains
Autor: Cerni, S.
Skoric, D.
Ruscic, J.
Krajacic, M.
Papic, T.
Djelouah, K.
Nolasco, Gustavo
Data: 2009
Editora: Springer
Resumo: Citrus tristeza virus (CTV) represents one of the major threats to citrus production worldwide. In the East Adriatic region, CTV symptoms are mostly absent due to traditional citrus grafting on trifoliate orange (Poncirus trifoliata), a CTV-tolerant rootstock. Therefore, the virus has been continuously spreading by the propagation of infected material. The genetic variability of CTV was studied on nineteen citrus samples, collected from orchards in the coastal region of Croatia, Montenegro and Albania, that previously tested positive by ELISA and immunocapture RT-PCR. Single-strand conformation polymorphism of the amplified coat protein gene demonstrated the presence of different CTV variants in each amplicon, while sequence analysis of cloned CP gene variants confirmed their clustering into six out of the seven phylogenetic groups so far delineated. Four of these groups include sequences of severe quick decline, seedling yellows and stem-pitting (SP) isolates, thought to be found only rarely in the Mediterranean region. Regardless of the lack of symptoms in the field, CTV isolates from the East Adriatic displayed high genetic variability and pathogenic potential, additionally confirmed by biological characterisation. The high percentage of mixed infections suggest the potential for further diversification and a greater risk of severe variants spreading into new areas.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6249
DOI: https://dx.doi.org/10.1007/s10658-009-9444-0
ISSN: 0929-1873
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.