In vitro cloning of Ficus carica L. adult trees

Nobre, J.; Romano, Anabela

http://hdl.handle.net/10400.1/6436

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Authors

Nobre, J.

Romano, Anabela

Abstract(s)

The present work reports in vitro studies carried out with two Portuguese cultivars of fig-tree: Berbera (a dried fig) and Lampa (a fresh fig). Procedures were developed to overcome difficulties associated with the establishment of adult material due to contaminants and exudation of phenolic compounds. An improved growth and development of the apical shoot-tips was obtained on Muriithi medium supplemented with 0.05 % PVP. The highest multiplication rate, 5.3 shoots per culture, every three weeks, was achieved on a similar basal medium supplemented with 2.2 mu M BA and devoid of NAA. The best rooting response (96.9 %) was observed on the medium containing 2.5 mu M IBA. Plantlets were successfully acclimatized and grown for three months in the greenhouse, and then field established for orchard production. Micropropagated plants produced fruits two years after field-establishment. These results are a promising step in the direction of in vitro cloning of valuable genotypes directly from field-grown plants and the conservation of plant genetic resources.