Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.1/6545
Título: Correction of the misclassification of species in the Portuguese collection of Cucurbita pepo L. using DNA markers
Autor: Rodrigues, R.
Veiga, I.
Marreiros, A.
Rocha, F.
Leitão, J. M.
Data: 2014
Editora: Cambridge University Press
Resumo: In this study, the genetic variability among 130 accessions of the Portuguese germplasm collection of Cucurbita pepo L. maintained at the Banco Portugues de Germoplasma Vegetal was assessed using AFLP (amplified fragment length polymorphism) and RAPD (random amplified polymorphic DNA) techniques for the identification of a genetically diverse core group of accessions for field phenotypic analysis. The surprisingly completely different molecular patterns exhibited by multiple accessions was later confirmed in the distribution of the putative C. pepo plants into two clusters drastically separated at a very low level of genetic similarity (DICE coefficient = 0.37). Additional analyses with RAPD and ISSR (inter single sequence repeat) markers and the introduction of standard genotypes of C. maxima L. and C. moschata L. into the analyses allowed the identification of multiple accessions of the last species wrongly included in the C. pepo collection. This study is a good example of the usefulness of DNA markers in the establishment and management of plant germplasm collections.
Peer review: yes
URI: http://hdl.handle.net/10400.1/6545
DOI: https://dx.doi.org/10.1017/S147926211400046X
ISSN: 1479-2621
Aparece nas colecções:FCT2-Artigos (em revistas ou actas indexadas)

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