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Browsing by Author "Biemans, R."

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  • Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi
    Publication . Archer, Margarida; Rodrigues, Maria Luisa; Aurélio, M.; Biemans, R.; Cravador, A.; Carrondo, Maria A.
    Cinnamomin (CIN) belongs to a family of 10 kDa proteins designated as elicitins. Some of these proteins induce a hypersensitive response in diverse plant species, leading to resistance against fungal and bacterial plant pathogens. CIN was crystallized by the vapourdiffusion method using either ammonium sulfate or polyethyleneglycol (PEG) as precipitants in solutions buffered at around pH 7. These crystals are isomorphous and belong to the triclinic space group, with unit-cell parameters a = 31.69, b = 36.99, c = 44.09 A Ê , = 76.86, = 84.41, = 80.26 . A frozen crystal diffracted X-rays beyond 1.45 A Ê resolution on a synchrotron-radiation source.
    2000Journal article Open access Show more
  • Identification of an elicitin gene cluster in Phytophthora cinnamomi and analysis of the necrotic activity of a purified recombinant beta-cinnamomin
    Publication . Duclos, J.; Aurélio, M.; Graca, J.; Coelho, A. C.; Fauconnier, A.; Jacquet, Alain; Bollen, A.; Cravador, A.; Biemans, R.; Godfroid, Edmond
    The oomycetous fungus Phytophthora cinnamoni plays and important role in the necrotic activity observed on feeder root of cork oaks (Quercus suber L.) and eucalyptus (eucalyptus marginata Donn. Ex. Sm.)trees reducing the capacity of these hosts to absorb water and nutrients.
    1998Journal article Open access Show more
  • Porcine D-amino acid oxidase: determination of the mRNA nucleotide sequence by the characterization of genomic and cDNA clones
    Publication . Jacobs, P.; Brockly, F.; Massaer, M.; Loriau, R.; Guillaume, J. P.; Ciccarelli, E.; Heinderyckx, M.; Cravador, A.; Biemans, R.; Van Elsen, A.; Herzog, A.; Bollen, A.
    Oligodeoxynucleotide probes derived from the published amino acid (aa) sequence for D-amino acid oxidase (DAO) [Ronchi et al. J. Biol. Chem. 259 (1982) 8824-8834] were used to screen cDNA libraries made from porcine kidney cortex and liver. Whereas no clones were obtained from kidney mRNAs, 20 independent ones were isolated from the liver library. Surprisingly, all of them carried only partial cDNAs for DAO starting around aa 100 in the coding sequence and extending for up to 250 bp in the 3'-noncoding sequence. One of these clones, pULB9103, was used to screen a porcine genomic library and allowed the isolation of DAO gene clone phULB001. Four exons encoding aa 1-151 were identified and sequenced, as well as the relevant exon-intron junctions. The mRNA sequence coding for DAO has been reconstituted from the genomic and cDNA sequences; its analysis by computer did not reveal and significant secondary structure, or particular feature, which could explain the failure to obtain full-length cDNAs.
    1987Journal article Restricted access Show more