Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi

Archer, Margarida; Rodrigues, Maria Luisa; Aurélio, M.; Biemans, R.; Cravador, A.; Carrondo, Maria A.

http://hdl.handle.net/10400.1/1202

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Authors

Archer, Margarida

Rodrigues, Maria Luisa

Abstract(s)

Cinnamomin (CIN) belongs to a family of 10 kDa proteins designated as elicitins. Some of these proteins induce a hypersensitive response in diverse plant species, leading to resistance against fungal and bacterial plant pathogens. CIN was crystallized by the vapourdiffusion method using either ammonium sulfate or polyethyleneglycol (PEG) as precipitants in solutions buffered at around pH 7. These crystals are isomorphous and belong to the triclinic space group, with unit-cell parameters a = 31.69, b = 36.99, c = 44.09 A Ê , = 76.86, = 84.41, = 80.26 . A frozen crystal diffracted X-rays beyond 1.45 A Ê resolution on a synchrotron-radiation source.

Keywords

Beta-cinnamomin Elicitins Phytopathogenic fungi

URI

http://hdl.handle.net/10400.1/1202

Citation

Archer, Margarida; Rodrigues, Maria Luisa; Aurélio, M.; Biemans, R; Cravador, Alfredo; Carrondo, Maria A.. Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi. Acta Crystallographica , 56, 3, 363-366, 2000.