Browsing by Author "Cabrita, L. F."
Now showing 1 - 2 of 2
Results Per Page
Sort Options
- Molecular markers in genetic variability assessment, cultivar fingerprinting and hybrid identification in fruit and vegetablesPublication . Leitão, José; Elisiario, P. J.; Cabrita, L. F.; Justo, E. M.; Pereira, G. M.; Carlier, Jorge; Farinho, M. J.Isozyme, RADP and AFLP markers were used in the UCTA, University of Algarve, for several studies in fruit tree and vegetable species. In Citrus, isozyme analysis was used for assessing genetic variability of traditional cultivars, for distinguishing nucellars from zigotics scion and rootstock cultivars, for parentage determination of hybrid plants and to identify mistakes occurring in commercial nurseries. In figs (Ficus carica L.), isozyme and RAPD analyses allowed the detection of errors in a field collection, and jointly with AFLP analysis were used to discriminate and fingerprint germplasm accessions. In almond (Prunus dulcis Webb) isozyme, RAPD and AFLP markers were utilised to discriminate among cultivars. Recently, several programs aiming to identify molecular markers linked to resistance genes and to construct genomic maps in pineapple, Brassica sp., peas and chickpeas, were initiated in our laboratory.
- Suitability of isozyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clonesPublication . Cabrita, L. F.; Aksoy, U.; Hepaksoy, S.; Leitão, J. M.Sarilop is the main and standard cultivar for commercial dried fig (Ficus carica L.) production in Turkey. Eleven of the most promising Sarilop clones and one clone of Sarizeybek, all selected from a former agronomic evaluation, were analysed by three molecular marker techniques, isozymes, RAPDs and AFLPs. The resolution power and the accuracy of these three analytical techniques, in distinguishing among fig clones, were determined. The analysis of five isozyme systems permitted the discrimination between the two cultivars, Sarilop and Sarizeybek. Besides the discrimination between the two fig cultivars, the use of 31 10-mer primers in RAPD analysis allowed splitting the 11 Sarilop clones into two groups of genetic similarity, but not to distinguish between all the clones. The AFLP(TM) technology showed a much higher multiplex ratio than the RAPD technique (42.4 vs. 6.1) and eight combinations of EcoR1/Mse1 primers were enough to clearly distinguish between all the Sarilop clones. (C) 2001 Elsevier Science B.V. All rights reserved.