Percorrer por autor "Calado, Beatriz Lopes"
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- Characterization of bacteria in seafood and environmental waters samples from Namibe (Angola)Publication . Calado, Beatriz Lopes; Cardoso, João; Power, Deborah MFoodborne diseases are a major burden worldwide and are very common and one of the major causes of death in underdeveloped countries. Coliform bacteria are a non-taxonomic group of Enterobacterales order that commonly inhabit the intestines of warm-blooded animals. While most coliform bacteria are not associated with foodborne illness, there are coliforms (faecal origin) that can cause diseases in humans of which most cases are associated with pathogenic E. coli strains. Therefore, detection of coliforms is commonly used as an indirect measure of quality control and safety of foods and waters for human consumption. The present study aimed to isolate and characterise coliform bacteria from bivalves that are used for human consumption and from their environmental seawater to identify species that may represent a potential risk for human health. Four different locations (Mucoio, Praia das Conchas, Sacomar, and Praia Amélia) in the coast of Namibe in Angola were sampled and microbiology techniques, molecular analysis, biochemical and virulence assays were performed to characterise the different isolates. Our results revealed that isolates correspond to Escherichia coli, Enterobacter spp., Klebsiella spp. and Citrobacter freundii and that the mussels collected from Praia das Conchas showed the highest fecal coliform contamination and diversity. At least 6 different strains of E. coli, 4 of Enterobacter spp. and 2 Klebsiella spp. and 1 of Citrobacter freundii were found and they show different resistance to cephalosporins and penicillins which are common antibiotics used in clinical. Virulence tests were performed using a representative strain from each species using the arthropod model Galleria mellonella and suggest that all strains are likely to be pathogenic and most or the larvae were death in the first 24h post injection. This study identify for the first time pathogenic coliforms bacteria in bivalve food samples and environmental waters from Namibe which may represent a health risk to the local population and the major causative agents of foodborne and other diseases.