Browsing by Author "Candeias, Rui"
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- Bradiarritmias: abordagem em contexto pré e intra-hospitalarPublication . Costa, Hugo; Azevedo, Pedro; Carvalho, Daniela; Candeias, Rui; Jesus, IlidioAs bradiarritmias são eventos arrítmicos frequentes em contexto urgente e emergente. A sua frequência aumenta com a idade devido à evolução degenerativa do sistema de condução cardíaco, embora também possam ser encontradas em idades jovens e atletas treinados. A doença sinoauricular e os bloqueios auriculoventriculares são os mecanismos fisiopatológicos mais comuns no que diz respeito aos distúrbios da condução. Uma correta investigação é essencial na identificação etiológica (intrínseca ou extrínseca ao tecido cardíaco) bem como na forma de instalação e no caráter de reversibilidade. As causas reversíveis devem ser prontamente corrigidas e são essenciais na estabilização do doente. Na presença de bradicardia, a apresentação clínica deve guiar a abordagem inicial, assim como a existência de sinais de gravidade no electrocardiograma. A terapêutica farmacológica e o pacing transcutâneo são medidas/atitudes intermédias de suporte hemodinâmico como ponte para intervenções especializadas. Assim, uma correta avaliação e rápida abordagem em ambiente pré-hospitalar ou hospitalar possibilita a estabilização do doente e posterior encaminhamento para estudo dirigido.
- Characterization of 15 polymorphic microsatellite loci in the temperate reef fish Lepadogaster lepadogaster, developed using 454-sequencingPublication . Teixeira, Sara; Candeias, Rui; Klein, Maria; Serrão, Ester; Borges, R.Abstract The clingfish, Lepadogaster lepadogaster is a reef fish species, abundant in temperate nearshore rocky reefs of the Eastern Atlantic and central and Eastern Mediterranean. To study genetic variability and population connectivity of this species, we developed fifteen polymorphic microsatellite markers. These were tested in one population and all but one, showed no departure from Hardy–Weinberg equilibrium. Average overall observed heterozygosity was 0.66 and allelic richness was 8.9. Two primer pairs revealed possible linkage disequilibrium. These markers open perspectives for population genetic studies of this species to unravel connectivity and population biology, vital information for future conservation studies.
- Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superbaPublication . Candeias, Rui; Teixeira, Sara; Duarte, C. M.; Pearson, G. A.Background: The Antarctic krill, Euphausia superba is a pelagic crustacean, abundant in high-density swarms (10 000 – 30 000 ind/m2) with a circumpolar distribution and a key role in the food web of the Southern Ocean. Only three EST derived microsatellite markers have been used in previous genetic studies, hence we developed additional highly polymorphic microsatellite markers to allow robust studies of the genetic variability and population differentiation within this species. Findings: The microsatellite markers described here were obtained through an enriched genomic library, followed by 454 pyrosequencing. A total of 10 microsatellite markers were tested in 32 individuals from the Antarctic Peninsula. One of the tested loci was fixed for one allele while the other was variable. Of the remaining nine markers, seven showed no departure from Hardy-Weinberg equilibrium. The mean number of alleles was 14.9. Conclusions: These markers open perspectives for population genetic studies of this species to unravel genetic structure, dispersal and population biology, vital information for future conservation.
- Congenital left ventricular apical aneurysm presenting as ventricular tachycardiaPublication . Amado, José; Marques, Nuno; Candeias, Rui; Gago, Paula; de Jesus, IlidioThe authors present the case of a 34-year-old male patient seen in our department due to palpitations. On the electrocardiogram monomorphic ventricular tachycardia (VT) was documented, treated successfully with amiodarone. The subsequent study revealed a normal echocardiogram and an apical aneurysm of the left ventricle on magnetic resonance imaging, confirmed by computed tomography coronary angiography that also excluded coronary disease.He underwent an electrophysiological study to determine the origin of the VT and to perform catheter ablation using electroanatomical mapping. VT was induced and radiofrequency applications were performed in the left ventricular aneurysm area. VT was no longer inducible, with acute success. Despite this it was decided to implant a subcutaneous implantable cardioverter-defibrillator (ICD). Eight months after the ablation the patient was admitted again due to VT, treated by the ICD. (C) 2016 Sociedade Portuguese de Cardiologia. Published by Elsevier Espana, S.L.U. All rights reserved.
- Fibrilhação auricular - uma abordagem pré-hospitalar “descomplicada”Publication . da Mota, Teresa Faria; Candeias, Rui; Mimoso, Jorge; de Jesus, IlidioA Fibrilhação Auricular (FA) é a arritmia crónica mais comum a nível mundial, com uma prevalência que poderá duplicar nas próximas décadas. O seu diagnóstico baseia-se na análise do ECG de 12 derivações e caracteriza-se habitualmente pela ausência de ondas p e pela irregularidade dos intervalos R-R, embora possam existir exceções. Uma das principais e mais nefastas complicações da FA são os eventos tromboembólicos cerebrais ou periféricos, o que sustenta a pertinência de estabelecer o risco trombótico individual de cada doente e iniciar anticoagulação de acordo com o mesmo.
- Lack of fine-scale genetic structure and distant mating in natural populations of Fucus vesiculosusPublication . Teixeira, Sara; Pearson, G. A.; Candeias, Rui; Madeira, Celine; Valero, Myriam; Serrão, EsterFine-scale spatial genetic structure (SGS) within populations reflects the dispersal behaviour of genes and individuals. Here we studied very small-scale SGS and mating patterns in the brown seaweed Fucus vesiculosus, a dioecious marine broadcast spawner with immediate settlement upon gamete release, which is predicted to strongly restrict gene flow. We estimated SGS, inbreeding and kinship for adults and recruits from habitats with contrasting exposures and patchiness (open coast and estuarine ecosystems) using microsatellite loci. Heterozygote deficiency was found for most adult populations but it was even higher for recruits, indicating inbreeding depression. At the fine spatial resolution of this study there was no spatial genetic structuring for 3 of the 5 populations studied across different habitats. Habitat could not explain the unrestricted gene flow in some populations. In the kinship analyses, we identified more putative mothers than fathers, suggesting that male gamete dispersal mediates gene flow at broader distances. However, the vast majority of the parents of the recruits could not be found among the adults sampled nearby, indicating unrestricted gene flow at these small scales. We propose 3 nonexclusive hypotheses for our findings: (1) unrestricted gene flow, (2) inbreeding depression eliminating most inbred individuals resulting from nearby related parents, (3) temporal Wahlund effects, mediated by a hypothetic genetic pool of a bank of microscopic forms persisting from previous generations.
- Microsatellite markers developed through pyrosequencing allow clonal discrimination in the invasive alga Caulerpa taxifoliaPublication . ARNAUD-HAOND, Sophie; Candeias, Rui; Serrão, Ester; Teixeira, SaraPolymorphic microsatellites were developed for the invasive green algae Caulerpa taxifolia using next generation DNA sequencing. Results showed a limited rate of microsatellites for the amount of sequences, possibly explaining failure of previous attempts for microsatellite development through classical methods. Eight polymorphic loci were selected that exhibited polymorphism and a null or negligible rate of amplification failure. The number of alleles per locus ranged from two to seven. The reconstruction of Multi Locus Genotypes and the heterozygosity and departure from Hardy–Weinberg equilibrium confirmed the influence of clonal reproduction and showed the usefulness of this set of markers to successfully discriminate clonal lineages and analyze the clonal and genetic composition of algal beds. These markers will be used to further investigate the clonal composition and genetic structure in populations of C. taxifolia and to attempt retracing the origin of and pathways followed by invasive clonal lineages.
- Polymorphic microsatellite markers in the brown seaweed Fucus vesiculosusPublication . Candeias, Rui; Casado-Amezúa, Pilar; Pearson, G. A.; Serrão, Ester; Teixeira, SaraBackground: Fucus vesiculosus is a brown seaweed dominant on temperate rocky shores of the northern hemisphere and, is typically distributed in the mid-upper intertidal zone. It is an external fertilizer that reproduces sexually, providing an excellent model to address conflicting theories related to mating systems and sexual selection. Microsatellite markers have been reported for several Fucus species, however the genomic libraries from where these markers have been isolated, have originated from two or more species pooled together (F. vesiculosus and F. serratus in one library; F. vesiculosus, F. serratus and Ascophyllum nodosum in a second library), or when the genomic DNA originated from only one species it was from Fucus spiralis. Although these markers cross-amplify F. vesiculosus individuals, the level of polymorphism has been low for relatedness studies. Findings: The microsatellite markers described here were obtained from an enriched genomic library, followed by 454 pyrosequencing. A total of 9 microsatellite markers were tested across 44 individuals from the North of Portugal. The mean number of alleles across loci was 8.7 and the gene diversity 0.67. Conclusions: The high variability displayed by these microsatellite loci should be useful for paternity analysis, assessing variance of reproductive success and in estimations of genetic variation within and between populations.
- Taquidisritmias supraventriculares:abordagem em contexto pré e intra-hospitalarPublication . Costa, Hugo; Azevedo, Pedro; Carvalho, Daniela; Candeias, Rui; de Jesus, IlídioAs taquidisritmias supraventriculares (TSV) são relativamente frequentes, constituem um grupo heterogéneo de arritmias e o seu diagnóstico diferencial é muitas vezes difícil na prática clínica. Apresentam clínica variável que depende da gravidade do evento arrítmico, das comorbilidades associadas bem como das características inerentes ao própriodoente. Embora a maioria das TSV se apresente com complexos QRS estreitos e as taquicardias ventriculares (TV) com complexos QRS largos, isto nem sempre acontece, sendo essencial uma cuidadosa avaliação para um diagnóstico correto.